Definition of peptide microarrays<\/strong><\/p>\n Peptide microarrays<\/a> (also commonly called peptide chips or peptide epitope microarrays) are designed based on protein-protein interactions, and a known protein array is immobilized on a carrier to detect paired unknown proteins. The protein immobilized on the carrier can be antibodies, antigens, receptors, ligands, enzyme substrates, and protein binding factors. This new technology can detect the relative abundance of hundreds of proteins in biological samples in a single experiment. Its core technologies include: design, modification, and high-purity synthesis of new peptide molecules that specifically bind to the target protein; high-precision, high-throughput peptide array chip design and synthesis of the target protein binding site; peptide and peptide-protein reactions detection system; antibody-specific epitope original discovery system; target protein binding peptide discovery system; and huge epitope element-binding sample library and peptide molecule sample library.<\/p>\n Peptide microarrays can effectively find disease-specific markers from patient biological samples and can be used for the early diagnosis of various diseases including tumors. At the same time, the use of peptide microarray technology can screen out effective substances from a large number of pharmaceutical ingredients, which is of vital significance for the design and development of new drugs and targeted individual treatment.<\/p>\n Determination guidelines for peptide microarrays<\/strong><\/p>\n The determination criteria for peptide microarrays are similar to the enzyme-linked immunosorbent assay (ELISA) protocol. The peptides (thousands of several copies) are attached to the surface of the glass chip. This peptide chip can be directly cultivated with various biological samples, such as purified enzymes or antibodies, patient or animal serum, cell lysates, etc. After some washing steps, a secondary antibody with the required specificity (for example, human\/mouse anti-IgG or anti-phosphotyrosine or anti-myc) is used. Typically, the secondary antibody is labeled with a fluorescent label<\/a> that can be detected by a fluorescent scanner. Other detection methods include chemiluminescence or autoradiography. By analyzing the differences in the results of microarray screening of patient samples and healthy human samples, we can obtain biomarkers that are highly related to the disease, and further use these biomarkers to assist in the diagnosis, monitoring, and treatment effect evaluation of the disease.<\/p>\n Features of peptide microarrays<\/strong><\/p>\n Application of peptide microarrays<\/strong><\/p>\n References:<\/strong><\/p>\n Definition of peptide microarrays Peptide microarrays (also commonly called peptide chips or peptide epitope microarrays) are designed based on protein-protein interactions, and a known protein array is immobilized on a …<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[157,158,156],"_links":{"self":[{"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/posts\/705"}],"collection":[{"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/comments?post=705"}],"version-history":[{"count":1,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/posts\/705\/revisions"}],"predecessor-version":[{"id":706,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/posts\/705\/revisions\/706"}],"wp:attachment":[{"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/media?parent=705"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/categories?post=705"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/tags?post=705"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\n
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