kalata B1<\/a>. Cyclic peptides display a wide range of pharmaceutically important biological activities, but their natural function is to act as insecticides in plant defense. In the structure of the cyclic peptide kalata B1, all 23 non-cysteine residues are successively substituted by alanine. The data is tested by nuclear magnetic resonance spectroscopy.<\/p>\nReferences<\/strong><\/p>\n\n- Morrison K L, Weiss G A. Combinatorial alanine-scanning[J]. Current opinion in chemical biology, 2001, 5(3): 302-307.<\/li>\n
- Weiss G A, Watanabe C K, Zhong A, et al. Rapid mapping of protein functional epitopes by combinatorial alanine scanning[J]. Proceedings of the National Academy of Sciences, 2000, 97(16): 8950-8954.<\/li>\n
- Simonsen S M, Sando L, Rosengren K J, et al. Alanine scanning mutagenesis of the prototypic cyclotide reveals a cluster of residues essential for bioactivity[J]. Journal of biological chemistry, 2008, 283(15): 9805-9813.<\/li>\n<\/ol>\n
\n- Howlader M T H, Kagawa Y, Miyakawa A, et al. Alanine scanning analyses of the three major loops in domain II of Bacillus thuringiensis mosquitocidal toxin Cry4Aa[J]. Applied and environmental microbiology, 2010, 76(3): 860-865.<\/li>\n
- Edelheit O, Hanukoglu I, Dascal N, et al. Identification of the roles of conserved charged residues in the extracellular domain of an epithelial sodium channel (ENaC) subunit by alanine mutagenesis[J]. American Journal of Physiology-Renal Physiology, 2011, 300(4): F887-F897.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"
Introduction Alanine scanning library is one of the peptide library design tools. Alanine Scanning Library, this tool allows you to systematically substitute alanine for each amino acid position in your …<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[162,163,50],"_links":{"self":[{"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/posts\/712"}],"collection":[{"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/comments?post=712"}],"version-history":[{"count":1,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/posts\/712\/revisions"}],"predecessor-version":[{"id":714,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/posts\/712\/revisions\/714"}],"wp:attachment":[{"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/media?parent=712"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/categories?post=712"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.creative-peptides.com\/blog\/wp-json\/wp\/v2\/tags?post=712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
![\"\"](\"https:\/\/www.creative-peptides.com\/blog\/wp-content\/uploads\/2021\/10\/Figure-1.-Example-of-Alanine-Scanning.-The-native-protein-top-row-and-each-possible-point-mutation-to-alanine-is-considered-300x248.png\")
<\/a><\/p>\n