High Resolution Conformational Epitope Mapping
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High Resolution Conformational Epitope Mapping

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Several methods are currently available for the analysis of antigenic epitopes: X-ray diffraction, phage presentation of random peptide libraries, LC-MS analysis, and 3D-EM analysis. The application of 3D-EM in biology has not only remained in a simple and intuitive description, but also has carried out a three-dimensional study from qualitative to quantitative, from plane to space. This is of great significance for understanding the spatial structure of biological macromolecules and their relationship with function. Among them, the method of X-ray crystal diffraction is the most accurate method, and the method of antigen-antibody co-crystallization can be accurate to the atomic level.

Schematic-Outline-of-the-Epitope-Mapping-Approach.png

Fig.1 Schematic Outline of the Epitope Mapping Approach (Najar, et al. 2017)

Analytical method

  1. HDX-MS

HDX-MS is a method for studying protein-ligand interactions and protein-protein interactions. The basic principle of this method is to immerse proteins in heavy water. The hydrogen on the surface of the protein in close contact with heavy water is faster than the hydrogen exchange rate inside the protein. Once the two proteins bind to each other, the solvent cannot reach the interface of its interaction. This principle is reflected in the hydroquinone exchange kinetics, and the amount of ruthenium contained in the antigen when bound or not bound to the monoclonal antibody can be obtained by mass spectrometry.

  1. X-ray-resolved monoclonal antibody-antigen complexes

X-ray-resolved monoclonal antibody-antigen complexes are the most reliable method for analyzing epitope recognition by monoclonal antibodies. The method is based on the intensity and angle measurement of the X-ray beam of the monoclonal antibody-antigen eutectic diffraction, and the bioinformatics method is used to determine the atomic coordinates. As a result, the three-dimensional structure of the complex is completely visualized.

  1. N62

N62 is a monoclonal antibody to Francisella tularensis. The X-ray crystal structure of the Fab segment of monoclonal antibody N62 shows that the antigen-binding region is composed of aromatic amino acids, and the aromatic amino acid forms a small recess containing no more than 4/3 sugar residues, indicating that N62 is mainly The Qui4NFm residue at the non-reducing end of the Franciamycin T antigen binds.

  1. Mnt C

Mnt C is a Staphylococcus aureus transporter. GRIBENKO et al. used X-ray crystallography to analyze the complex of Mnt C and its monoclonal antibody 305-78-7. It is shown that 305-78-7 binds to the C-terminal leaflet of Mnt C, and α-helixα8 and β-sheetβ8 constitute the major interface of the antigen-antibody. In addition, E247 of Mnt C may form a salt bridge with K58 on the heavy chain of the antibody.

Our Services

Creative Peptides provides a comprehensive series of Epitope Mapping Services to fulfill customers’ demand. Our Creative MapTM Platform is developed to map various epitopes flexibly. Based on the three-dimensional electron microscopy technology, the epitope analysis technique requires only a small amount of antigen/antigen complex to be completed within quickly. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you. Please be free to contact us.

References:
1. Najar, T. A., Khare, S., Pandey, R., Gupta, S. K., & Varadarajan, R. (2017). Mapping protein binding sites and conformational epitopes using cysteine labeling and yeast surface display. Structure, 25(3), 395.
2. Lu Z, Rynkiewicz MJ, Yang CY, Madico G, Perkins HM, & Wang Q, et al. (2013). The binding sites of monoclonal antibodies to the non-reducing end of francisella tularensis o-antigen accommodate mainly the terminal saccharide. Immunology, 140(3), 374-389.
3. Gribenko, A. V., Parris, K., Mosyak, L., Sheng, L., Handke, L., & Hawkins, J. C., et al. (2016). High resolution mapping of bactericidal monoclonal antibody binding epitopes on staphylococcus aureus antigen mntc. Plos Pathogens, 12(9), e1005908.

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