Macrocyclic Peptides Synthesis

Linear Peptide Synthesis: Using Fmoc solid-phase peptide synthesis (SPPS) method, we can synthesize small molecule linear peptides containing 2-50 amino acids with a purity of over 95%.

Cyclic Peptide Synthesis: We offer the synthesis of various types of cyclic peptides, including:

Amide Bond / Lactone Bridge Cyclic Peptides: Cyclic peptides are formed by creating amide bonds at different positions within the peptide, enabling various cyclic forms such as head-to-tail or side-chain-to-side-chain connections.

Disulfide Bond Cyclic Peptides: Typically formed between cysteine residues, this is one of the most common cyclic forms found in nature and can be applied in drug discovery and other fields.

Thioether Cyclic Peptides: Using reagents such as chloroacetic acid and 1,4-bis(bromomethyl)benzene to selectively react with cysteine residues, resulting in peptides with rigidity and enhanced target binding ability.

Stapled Peptides Synthesis: Stabilizing the α-helix structure through ring-closing metathesis (RCM) reactions, using specific building blocks and positioning.

Bicyclic Peptides: Peptides with two chemical constraints in their rings, typically synthesized using trivalent reagents like TBMB or TATA, capable of targeting two receptors simultaneously.

Click Chemistry Cyclic Peptides: Conducted on unprotected peptide side chains, the resulting bonds are stable and easy to handle, suitable for large-scale synthesis.

Amino Acid Modification: Modifications can be made to the N-terminus, C-terminus, or side chains of peptides, including but not limited to biotin labeling, fluorescent labeling, PEGylation, phosphorylation, glycosylation, isoleucine modification, and more.

Incorporation of Non-Natural Amino Acids: We can incorporate various non-natural amino acids, such as azido-AAs, N-methyl amino acids, homo-amino acids, etc., into macrocyclic peptides, imparting special functions and properties to the peptides.

Special Group Conjugation: We can conjugate macrocyclic peptides with specific chemical groups, drug molecules, fluorescent dyes, biotin, PEG, lipids, etc., to enable functions like drug delivery, imaging, diagnostics, and more.

Peptide Conjugation Services: This includes metal chelation conjugation (RDC), peptide-drug conjugation (PDC), and other services. We can also synthesize peptide-linker heads for subsequent antibody bioconjugation, aimed at developing antibody-drug conjugates (ADCs).

Synthesis Scale Customization: We offer custom synthesis services ranging from microgram-scale (10-150 nmol) to gram-scale (>1g) and even kilogram-scale synthesis based on customer requirements.

Purity Customization: We can synthesize crude peptides and provide high-purity macrocyclic peptides with purities exceeding 95%, 98%, or more, as required.

Salt Form Customization: We can provide peptides in various salt forms, including TFA, acetate, HCl, and others.

Special Requirement Customization: We can provide specific packaging forms, storage conditions, solvent systems, etc., and also offer customized sequence design and synthesis of non-natural amino acids based on customer needs.

Quality Analysis: Equipped with advanced analytical instruments like high-resolution LCMS, we conduct rigorous quality testing on synthesized peptides, including purity analysis, molecular weight determination, amino acid composition analysis, etc., to ensure product quality meets the required standards.

Purification Services: With various purification equipment and techniques, we can use methods such as liquid chromatography to purify macrocyclic peptides, improving the purity and quality of the final products.

Process Development and Optimization: With rich experience and expertise, we provide process development and optimization services for macrocyclic peptide synthesis, including synthesis route design, reaction condition optimization, and purification process improvement to enhance synthesis efficiency and product quality.

Stability Studies: We offer stability studies for synthesized macrocyclic peptides, including stability tests under different temperature and pH conditions, as well as long-term storage stability studies, providing scientific evidence for product preservation and transportation.

Activity Assessment and Screening: Using cell-based screening models, animal models, and other methods, we evaluate and screen the activity of macrocyclic peptides, helping customers quickly identify potential compounds with practical applications.