Prenylation (also known as isoprenylation or lipidation) is one of the common post-translational peptide modifications, which involves the addition of a farnesyl (C15) or geranylgeranyl (C20) isoprenoid moiety onto a cysteine residue located near the carboxyl terminal of a peptide. These chemical reactions were catalyzed by farneyl transferase (FTase) and geranylgeranyltransferase (GGTases), respectively. Proteins modified by isoprenylation play an important role in signal transduction in vivo. The role of Ras superfamily guanosine triphosphaterase (GTPases) in tumorigenesis and development has attracted wide attention.
Application of Peptide Prenylation
Since discovered in 1978, prenylation has been studied extensively due to its various aspects of cellular physiology and pathology processes by increasing the hydrophobicity of the target protein for efficient anchoring into plasma membranes and/or organellar membranes. These target proteins include the small GTPases in the Ras, Rho, and Rab families, heterotrimeric G proteins, and nuclear lamin proteins. Functions of prenylation are involved in facilitating subcellular localization, promoting protein-protein and protein-membrane interactions. However, the dysregulation of prenylation drives to many severe human diseases such as cancer, cardiac effects, neurodegenerative disorders, retinitis pigmentosa, and premature aging syndromes.
Creative Peptides has developed a reliable procedure for the synthesis of prenylated peptides, we can provide global customers with prenylated peptides based on our excellent technology. It includes farnesylation and geranylgeranylation. Prenylated C-terminus peptide was conjugated to Keyhole Limpet Hemocyanin (KLH), and purified by affinity chromatography.
Creative Peptides has extensively developed prenylated peptides structures and is the company of choice to manufacture your prenylated peptides requirements, providing a confidential and efficient service at competitive prices. Every step of peptide synthesis is subject to Creative Peptides’ stringent quality control. Typical delivery specifications include:
- HPLC chromatogram
- Mass spec analysis
- Synthesis report
- Certificate of Analyses
1. Gelb, M. H., Scholten, J. D., & Sebolt-Leopold, J. S. (1998). Protein prenylation: from discovery to prospects for cancer treatment. Current opinion in chemical biology, 2(1), 40-48.
2. Xie, F., Li, P., Gong, J., Zhang, J., & Ma, J. (2015). The bisphosphonate zoledronic acid effectively targets lung cancer cells by inhibition of protein prenylation. Biochemical and biophysical research communications, 467(4), 664-669.