Peptidomimetics Service
Creative Peptides delivers expert services in peptidomimetics focused on creating and synthesizing peptidomimetic compounds to address natural peptide limitations during drug development. Our service enables the creation of stable peptidomimetics with high bioavailability tailored to client specifications while offering comprehensive support through biological testing and structural refinement services. Our service portfolio extends across several domains including drug discovery and protein-protein interaction (PPI) inhibitor development while supporting biomedical research to facilitate faster product development and scientific exploration for our clients and delivering cutting-edge diagnostic and therapeutic solutions.
Our Peptidomimetic Services
01Peptidomimetic Design and Synthesis
Customized Design: Using the client-provided target protein structure or functional requirements we engineer both the amino acid sequence and the 3D structure of peptidomimetics to achieve high affinity and specificity binding to the target protein.
Diverse Modifications: Our modification strategies include multiple techniques such as:
D-amino acid substitution: Enhances the peptidomimetic's resistance to proteases.
Cyclization modifications: Cyclization modifications create cyclic structures that enhance both stability and bioavailability.
Backbone substitution: The backbone of natural peptides is substituted with non-natural backbone structures like β-amino acids and γ-amino acids to improve stability.
Side-chain modifications: The addition of specific side-chain groups enables the optimization of peptidomimetics' pharmacokinetic properties.
02Biological Activity Testing
In vitro Activity Testing: Assess the binding ability of the peptidomimetics to the target protein, including:
Enzyme activity inhibition test: Measures the inhibitory effect of the peptidomimetics on specific enzyme activities.
Fluorescence polarization assay: Determines the binding affinity of the peptidomimetics to the target protein.
Surface plasmon resonance (SPR): Real-time monitoring of the interaction dynamics between peptidomimetics and target proteins.
Cell-based Testing: Evaluate the functionality of peptidomimetics in a cellular environment, including:
Cell proliferation inhibition assay: Assesses the effect of peptidomimetics on the proliferation of specific cell lines.
Cell apoptosis induction assay: Evaluates the ability of peptidomimetics to induce apoptosis in cells.
Cell permeability assay: Measures the uptake efficiency of peptidomimetics in cells.
03Drug Screening
Peptidomimetic Library Construction: Compile a diverse library containing multiple peptidomimetic compounds that exhibit different structural forms and functional properties for the purpose of high-throughput screening.
High-Throughput Screening (HTS): Automated platforms should be used to rapidly evaluate the peptidomimetic library and find potential bioactive compounds.
Target Validation: Demonstrate the interactions between peptidomimetics and their target proteins by employing different experimental techniques including co-immunoprecipitation and mass spectrometry.
04Structural Optimization
Structure-Based Optimization: Apply X-ray crystallography and nuclear magnetic resonance (NMR) methods alongside additional techniques to examine the intricate structures of peptidomimetics and target proteins which facilitates structural optimization.
Computational Simulation: Employ molecular dynamics simulations along with virtual screening and additional computational approaches to determine binding modes and refine peptidomimetics.
Iterative Optimization: The stability and bioactivity of peptidomimetics improve continuously through repeated design-synthesis-testing-optimization cycles.
05Custom Services
Personalized Solutions: Provide a full-range custom service from initial design to final delivery, based on the specific needs of the client.
Special Request Support: Provide professional technical support and solutions for special requirements (such as specific modifications, unique targets).
06Quality Control
Purity Testing: Apply high-performance liquid chromatography (HPLC) techniques to maintain a high purity in peptidomimetics.
Structural Verification: Employ mass spectrometry (MS) techniques to confirm both the molecular structure and modification accuracy of peptidomimetics.
Stability Testing: Evaluate peptidomimetics stability across different environmental conditions to guarantee consistent performance in storage and application.
Service Advantages
- High Stability
Peptidomimetics exhibit improved resistance to proteases and extended half-life through structural changes such as cyclization and backbone substitution which solves stability problems seen in natural peptides.
- Optimized Pharmacokinetic Properties
Peptidomimetics address natural peptide pharmacokinetic limitations through enhanced oral bioavailability and improved cellular permeability.
- High Specificity and Affinity
Peptidomimetics replicate natural peptide three-dimensional structures which results in superior target protein binding specificity and affinity while minimizing unintended interactions.
- Customized Design
Our company specializes in customized peptidomimetic design and synthesis services that match specific biological or drug development requirements according to customer specifications.
- Diverse Applications
Drug discovery and protein-protein interaction inhibitor development among other areas benefit from the widespread application of peptidomimetics in biomedical research.
- Fast Delivery
Our advanced synthetic methods combined with efficient production processes enable us to deliver high-quality peptidomimetic compounds quickly.
- Technical Support
Our comprehensive technical support program offers design recommendations and biological activity testing along with structural optimization and additional services to assist clients with their development challenges.
- Strict Quality Control
Each peptidomimetic batch undergoes rigorous testing through advanced analytical methods including HPLC and MS to meet established quality standards.
Service Process
01Requirement Communication
03Synthesis and Purification
04Biological Activity Testing (Optional)
05Structural Optimization (Optional)
Clients submit their specifications for peptidomimetic design and synthesis which detail target proteins, biological functions and required modifications.
We develop the peptidomimetic structure by analyzing client requirements which includes determining amino acid sequences and modification strategies like cyclization and D-amino acid substitution. ).
Peptidomimetic compounds undergo synthesis through solid-phase or liquid-phase techniques and then undergo purification before verification through HPLC and MS alongside additional methods.
The biological activity testing of synthesized peptidomimetics measures their binding ability to target proteins and evaluates their stability and cellular permeability along with other factors.
Testing results lead to optimization of the peptidomimetic structure to increase its performance capabilities.
Clients receive high-quality peptidomimetic compounds together with their related technical reports and support services.
Peptidomimetic Applications
Drug Discovery
Peptidomimetics are crucial to drug discovery because they replicate natural peptides' three-dimensional structures and functions yet overcome issues of protease sensitivity alongside poor bioavailability and short lifespan. The design and synthesis of peptidomimetics leads to increased drug stability, bioavailability, receptor selectivity and potency thus speeding up drug discovery and development.
Protein-Protein Interaction (PPI) Inhibitor Development
Protein-protein interactions (PPIs) participate in numerous biological processes and contribute significantly to the development of multiple pathological conditions. Peptidomimetics replicate natural peptides' secondary structures including α-helices, β-sheets, and β-strands to obstruct protein interactions. Peptidomimetics show significant potential for PPI inhibitor development which can be particularly impactful for cancer diagnosis and treatment.
Biomedical Research
Peptidomimetics serve multiple purposes in biomedical research which includes examining protein functions and structures as well as their disease-related roles. Peptidomimetics function as investigative tools to examine the folding and stability of proteins and operate as inhibitors to study specific cell protein functions. The usage of peptidomimetics extends to creating innovative diagnostic instruments and treatments which include the development of peptidomimetic vaccines and drug delivery systems.
FAQ
How do you design peptidomimetics based on the target protein?
The amino acid sequence and 3D structure of the peptidomimetic are designed to match the structure and function of the target protein which results in high binding affinity and specificity. We examine the stability and bioavailability of the peptidomimetic to select suitable modification methods including D-amino acid substitution or cyclization.
How do you choose the appropriate modification strategy?
We determine modification strategies based on target protein characteristics and peptidomimetic application requirements. The decision to enhance stability may involve using either D-amino acid substitution or cyclization while improving bioavailability could require backbone substitution or side-chain modification.
How is the biological activity of peptidomimetics tested?
Our biological activity testing selection includes multiple in vitro assays (including enzyme activity inhibition tests, fluorescence polarization assays, and surface plasmon resonance among others). Our evaluation of peptidomimetics includes cell-based assays which measure cell proliferation inhibition, apoptosis induction and cellular permeability to analyze binding affinity and stability with target proteins.
What role do peptidomimetics play in protein-protein interaction (PPI) inhibitor development?
Peptidomimetics can mimic the secondary structures of natural peptides, such as α-helices and β-sheets, to block protein-protein interactions. This gives peptidomimetics significant potential in developing PPI inhibitors, particularly in the fields of cancer diagnosis and treatment.
