Bioconjugation is a chemical strategy to form a stable covalent link between two molecules, at least one of which is a biomolecule. The field of bioconjugation has had a considerable impact on academic research, clinical diagnostics and the production of therapeutics.
Peptides are an important type of molecules for derivatization by bioconjugation. Peptide–drug conjugates hold a promising stance in the delivery of therapeutic agents by providing distinct advantage of improving therapeutic potential of drugs. Both linear and cyclic peptides have been explored as trafficking moiety due to ease of synthesis, structural simplicity, and low probability of undesirable immunogenicity.
Targeted drug delivery
Research is ongoing for the development of new drug delivery systems for targeted drug delivery. Peptides derived from sequence of cell surface proteins have shown potent binding affinity to the target cell surface receptors. Equipping peptides-drug conjugates with target cell specific ligands like EGF and RGD peptides can provide a solution for selective and targeted delivery.
Peptide Biconjugation Service Descriptions
Creative Peptides provides a one-stop solution for all of your peptide-drug bioconjugation needs. Our custom peptide synthesis, modification, and conjugation services involve chemical modification of peptide at highly specific sites. This allows for the elegant attachment of drugs or bioactive molecules though a wide variety of coupling techniques
Creative Peptides gives you the best quality in peptides bioconjugation chemistry in the industry by implementing our state-of-the art peptides synthesis platform. Peptide synthesis, modifications, and conjugates produced at Creative Peptides have substantial purity. Each peptide-drug conjugate is meticulously monitored during synthesis and controlled according to our quality assurance and quality control standards
Coupling of drug and bioactive molecule with chemical reactive groups such as amine, thiol, carboxylate, hydroxyl, aldehyde and ketone, active hydrogen, photo-chemical and cycloaddition reactions, zero-length cross-linking, homobifunctional, heterobiofunctional, or multifunctional cross-linking chemistries, dendrimer and dendrons, and cleavable reagent systems.
Bioconjugate chemistry may occur through the C- and N-terminals of each polypeptide chain, the carboxylate groups of aspartic and glutamic acids, the ϵ-amine of lysine, the guanidino group of arginine, the sulfhydryl group of cysteine, the phenolate ring of tyrosine, the indol ring of tryptophan, the thioether of methionine, and the imidazole ring of histidine.
Peptide and amino acids
• Amino acids: Asp, Glu, Lys, Arg, Cys,Tyr, Trp, Met, His
• Synthetic peptides
Drug and bioactive molecule
All custom synthesis of biomolecules, modification, or bioconjugation services are manufactured under strict quality control processes. Analytical HPLC and MS analyses are performed in every development cycle. Final target conjugates must first be isolated from excess or unreacted reagent. In many cases, simple dialysis may suffice to remove unreacted reagent from the reaction solution. HPLC may also be used to either remove excess reagent or to isolate and characterize the cross-linked product. The cross-linked target molecule may then be further characterized by biochemical or biophysical techniques. QC (quality control) and QA (quality assurance) procedures are also followed independently to ensure the highest quality possible of every delivered conjugate.
Delivery Specifications: The typical delivery consists of lyophilized sample in individual fully labeled vials. The shipment also contains COA, MS, HPLC and/or other analytical data. Additional analytical data is also available upon request.