Peptide Chip Screening Platform

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).

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Creative Peptides is a leading biopharmaceutical company, focusing on the development and application of peptide chip screening platforms, committed to providing first-class peptide screening and related services to scientific research and medical institutions around the world.

The peptide on a chip screening platform is a cutting-edge biotechnology solution designed for high-throughput analysis of complex interactions between proteins and peptides. It integrates advanced peptide on a chip technology with supporting instruments such as the Aurora VERSA 110 peptide spotter. Designed to accelerate and optimize the execution of complex biological experiments. This innovative platform has demonstrated inestimable application potential and value in many frontier fields such as drug development, biomedical research and even disease diagnosis.

Preparation and application of whole proteomic peptide microarray of SARS-CoV-2

Peptide chip screening platform technical basis

Peptide chip technology

The core is the construction of a microscopic matrix containing thousands of uniquely sequenced peptides, which are sequentially anchored to a solid substrate (such as glass, microbeads, or microfluidic chips) to form a dense and ordered peptide lattice. Each point acts as a highly specific detection element capable of capturing and identifying the target molecule it matches, revealing a complex network of molecular interactions.

High throughput screening technology

Relying on the rapid development of automation and miniaturization technology, the platform can complete the interaction test of tens of thousands of peptides and a variety of target molecules (including proteins, antibodies, small molecules, etc.) at one time, greatly improving the screening efficiency and scope, and opening up new dimensions of speed and scale for scientific research.

Application of peptide chip screening platform

Antigen epitope screening

In this field, peptide chips play a key role by building a peptide library rich in potential epitopes, which can quickly lock the sequence matching a specific antibody, greatly shorten the antibody development cycle, and provide strong support for vaccine research and development, diagnostic kit innovation and basic immunology research.

Drug development and candidate molecular screening

Peptide chip technology shines in the field of drug discovery. It can efficiently screen high-affinity peptide candidates for specific protein targets. The combination of SPR and other biophysical technologies greatly improves the accuracy and efficiency of screening, and accelerates the incubation process of new therapeutic drugs.

Innovation in clinical testing and disease diagnosis

The platform provides rapid and precise analytical tools for clinical use to effectively diagnose diseases, monitor disease progression, and evaluate treatment effects by detecting changes in the activity of specific proteins, especially in the development of diagnostic reagents for cancer, autoimmune diseases and other fields.

An in-depth exploration of protein interactions

Peptide chips have demonstrated an extraordinary ability to systematically reveal protein-protein interaction networks, providing a detailed molecular map for understanding cell signaling, metabolic regulatory mechanisms, and disease pathology, and promoting a deeper understanding of complex phenomena in the life sciences.

Refinement of antibody discovery and optimization

In addition to its application in the study of antigen epitopes, peptide chips also promote the precise discovery and performance optimization of antibodies, and open up new ways to optimize the specificity and affinity of therapeutic antibodies by identifying the specific binding domain of antibodies.

A booster for basic scientific research

In the vast field of basic science research, peptide chip technology provides an efficient tool for exploring protein structure and function, post-translational modification and dynamic changes in physiological and pathological states, helping scientists to make breakthroughs in deep-seated problems in life science.

Our peptide chip screening services

Customized peptide chip services

Design and synthesize sequence-specific peptides according to customer requirements, covering a wide sequence space for screening specific protein targets.

Peptide sequences are synthesized using solid phase synthesis techniques such as Fmoc or t-Boc strategies. Support different length, different types of peptide sequences, including linear peptides, cyclic peptides, modified peptides, etc., to meet different research needs.

To ensure the purity of peptides, for advanced applications such as drug screening, purity requirements of 95% or more.

The synthesized peptide was fixed to the surface of the chip to form a peptide array. For example, using technologies such as lithography, inkjet printing, or point-of-sample robots.

Target interaction screening

incubation of drug targets of interest (such as receptors, enzymes, and transporters.) or parts of their structures (such as antibodies, ligand-binding domains) with peptide chips. The binding event of the peptide to the target is monitored and quantified by labeling the target (e.g., fluorescent labeling, radio labelling) or by utilizing label-free detection techniques (e.g., surface plasmon resonance, mass spectrometry).

Data analysis

After screening, data were collected to analyze which peptides showed the strongest binding affinity and specificity. Bioinformatics tools were used to further analyze the sequence characteristics of these peptides, including conserved sequences, structural features, and possible functional domains, to infer their drug potential.

Chemogenic evaluation

Further chemogenic evaluation of the selected peptides, including evaluation of stability and metabolic stability, cell penetration, toxicological properties, and production costs. These assessments help determine which candidate molecules are best suited as candidates for drug development.

Optimization and validation

Based on the results of preliminary screening and drug evaluation, the structure of potential peptides is optimized, such as by mutation, chemical modification and other methods to improve their activity, selectivity or pharmacokinetic properties. The optimized peptides need to be further validated for their bioactivity and safety through in vitro and in vivo experiments.

Why choose Creative Peptides?

High throughput screening capability: The technology can perform thousands of peptide screening experiments on a small chip, significantly improving the screening efficiency, reaching a per capita screening rate of 150,000+ solid compounds per day, which is more efficient than traditional screening methods.

High sensitivity: The biosensor used has high sensitivity and can accurately detect trace peptide drugs, which increases the sensitivity and possibility of drug research.

High specificity: Through precise bioinformatics design, the target peptide drug can be specifically identified and screened, reducing the false positive rate and improving the screening accuracy.

Convenient operation: The experimental operation process is simplified, and the laboratory personnel can complete the screening without professional experimental skills, and the operation threshold is lowered.

No resolution: Compared with hybrid library screening, peptide chip screening does not require complex resolution and resolution steps, and can directly obtain the screening results, which is more accurate and efficient.

Diversified molecular library: Our physical peptide library contains more than 2.4 million peptide compounds, diverse sources, extensive chemical space coverage, can provide a rich variety of peptide library selection, including full coverage library, cyclic peptide library, natural peptide library, non-natural peptide library, drug peptide library, etc., to meet different screening needs.

Rapid response to needs: Sub-libraries can be quickly selected or created according to the specific needs of each screening, flexibly responding to various research directions, and promoting the production of original peptide active molecules.

Technical support and services: Provide standardized operating processes, modular screening, customized services and comprehensive technical support to help customers move projects forward quickly, even inexperienced R&D teams can work efficiently.

FAQ

1. What is a peptide chip screening platform and how does it work?

A peptide chip screening platform is a high-throughput technology that synthesizes or immobilizes a large number of peptides on a solid substrate (such as a glass slide or membrane) to form a microarray. It is used to detect interactions between peptides and other biomolecules. The typical steps include peptide synthesis/immobilization, sample incubation, detection, and data analysis.

2. What are the main applications of peptide chip screening platforms?

Peptide chip screening platforms are widely used in antibody epitope mapping, T-cell epitope discovery, protein-peptide interaction studies, drug target screening, and biomarker discovery.

3. What is the process for screening peptide drugs using a peptide chip screening platform?

  • Designing and synthesizing peptides.
  • Immobilizing peptides on the chip surface.
  • Incubating the target molecules (such as proteins or small molecule drugs) with the peptide chip.
  • Using detection methods (such as fluorescence, radioactivity, spin labeling, or mass spectrometry) to detect interactions.
  • Analyzing data to identify potential peptide drugs.

4. Can peptide chip screening platforms detect interactions between peptides and small molecule drugs?

Yes, peptide chip screening platforms can be used to detect interactions between peptides and small molecule drugs, helping to discover and optimize potential peptide drug targets and mechanisms of action.

5. How long does it take to use a peptide chip screening platform?

The specific duration depends on the complexity of the project and the number of peptides. Generally, preparing the peptide chip, sample incubation, and detection can take from a few days to several weeks.

6. What detection methods are used in peptide chip screening platforms?

Common detection methods include fluorescence labeling, radioactive labeling, spin labeling, and mass spectrometry. The choice of detection method depends on the specific experimental requirements and sample characteristics.

7. What is the cost of using a peptide chip screening platform for peptide drug screening?

The cost depends on the level of customization of the peptide chip, the number of peptides screened, the detection methods used, and the specific project requirements. Generally, peptide chip screening platforms have high initial equipment and operational costs, but they significantly improve screening efficiency and accuracy.

8. How sensitive is the peptide chip screening platform?

Peptide chip screening platforms are highly sensitive and capable of detecting low-concentration biomolecular interactions, making them particularly suitable for screening and optimizing peptide drugs.

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).
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