Peptide-enzyme Conjugation

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).

Online Inquiry


Peptides are a kind of promising molecules with the potential protein-anchoring/modulating property, due to their large chemical diversity and the existence of well-established methods for library synthesis. There is a growing realization that it is possible to create synthetic structures with affinities and specificities comparable to natural antibodies, by using peptides as building blocks.

Enzymes often detect proteins by direct or indirect antibody detection strategies or as nucleic acid hybridization probes in sensitive assays. Reported enzyme molecules are widely used in biology due to their special properties. Although the detection of fluorescent and luminescent tags has made great progress, but enzyme conjugates can detect immune complexes by visual or spectrophotometry as sensitive probes. Enzyme labels provide the best overall performance, high sensitivity, low background, and can achieve fast detection.

Horseradish peroxidase and alkaline phosphatase are commonly used to linked to peptides or proteins.

For the detection of proteins, horseradish peroxidase (HRP) substrates are designed to produce signals for chromogenic, chemiluminescent or fluorescent oxidation. HPR has a molecular weight of 40,000. This molecular weight is lower than other enzyme conjugates, allowing for greater tissue and cell permeability and reducing the possibility of interfering with protein coupling.

Synthetic peptide-alkaline phosphatase conjugates, which was developed with alkaline phosphatase (AP) as the detector enzyme, permits epitopespecific analysis of antibody responses in experimentally or clinically derived lymphoid tissue.

Synthesis Stratagies

Two reaction mechanisms are involved in peptide-enzyme conjugation:

Peptide-enzyme Conjugation Fig.1 Reaction mechanisms of Peptide-enzyme Conjugation (Xu et al. 2018)

Creative Peptides offers custom peptide-enzyme coupling and modification services, as well as services such as experimental method development and testing for our customers. Our strengths in enzymatic engineering and peptide manufacturing will provide you with a specialized peptide-enzyme conjugate custom synthesis service, customizing proprietary peptide-enzyme conjugate products using different coupling methods.


  1. Fu, J., Reinhold, J., & Woodbury, N. W. (2011). Peptide-modified surfaces for enzyme immobilization. PLoS One, 6(4), e18692.
  2. Iwasa, S., Yoshida, I., & Kondo, K. (1985). U.S. Patent No. 4,517,290. Washington, DC: U.S. Patent and Trademark Office.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.

Customer Support & Price Inquiry


Creative Peptides is a custom lab chemical supplier that provides a wide range of peptides for the pharmaceutical and biotechnology industries.

Learn More

MEN 11270, a peptide antagonist at the human B2 kinin receptor

 MEN 11270 (H-DArg-Arg-Pro-Hyp-Gly-Thi-c(Dab-Dtic-Oic-Arg)c(7γ-10α)) is a novel selective constrained peptide ant ...

Function of dulaglutide in type 2 diabetes

 Dulaglutide, sold under the brand name Trulicity, is a GLP-1 receptor agonist which is a class of medications th ...

The Function of Darifenacin Hydrobromide in over Active Bladder

 Studies on the binding affinities of darifenacin hydrobromide and human muscarinic receptor subtypes show strong ...

The opiate delta receptor antagonist-ICI 154,129

ICl 154,129 is a new compound that shows selectivity as an antagonist of [Leu5]enkephalin and [D-AIa2, D-Leu5]en ...

Compstatin: a pharmaceutical inhibitor of complement

 Compstatin is a 13-residue cyclic peptide (Ile1-Cys2-Val3-Val4-Gln5-Asp6-Trp7-Gly8-His9-His10-Arg11-Cys12-Thr13- ...

Contact Us


Tel: |


Copyright © 2022 Creative Peptides. All rights reserved.