* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).
Surface Plasmon Resonance imaging (SPRi), namely surface plasmon resonance microscopy (SPRM), is a real-time, label-free, and high-throughput technique which is used to study biomolecular interactions based on detecting the refractive index changes resulting from molecular binding. More specifically, SPRi has been developed to examine affinity between biomolecules, screen biomarkers and detect biopsy specimens.
Surface Plasmon Resonance (SPR) is an optical detection process that occurs when a polarized light hits a prism covered by a thin planar metal (typically gold or silver) layer. At certain angles of incidence, a portion of the light energy couples through the gold coating and creates a surface plasmon wave at the sample and gold surface interface. The angle of incident light required to sustain the surface plasmon wave is very sensitive to changes in refractive index at the surface (due to mass change), and it is these changes that are used to monitor the association and dissociation of biomolecules.
Kinetic characterization by Surface Plasmon Resonance and other methods has already been an important step for drug researchers to select and characterize novel therapeutics as well as for basic life scientists to investigate specific biological binding events. When combined with appropriate surface chemistry, microfluidics and software, SPRi is unmatched in its range of applications including:
In conclusion, SPRi is a well-established leading technology for measuring binding association (ka) and dissociation rates (kd), affinities (KD). Creative Peptides offers SPRi (Surface Plasmon Resonance imaging) services including Biochip design and printing, Bio-interactions analysis (binding affinity and kinetic processes detection), Summary and analysis of the results. Typical SPRi services include:
As for our clients, all you need to do is just tell us the essential details of the experiment, and then our scientists will provide a comprehensive solution to match their scenario. At the end of the service they will get the result of the SPRi and the full experiment data.
References
Creative Peptides has accumulated a huge library of peptide knowledge including frontier peptide articles, application of peptides, useful tools, and more!
Pep2m, a peptide inhibitor of GluA2 subunit binding to NSF, reduces α-amino- 3-hydroxy-5-methyl-isoxazolepropion ...
Ziconotide (previously called SNX-111), currently marketed under the brand name of Prialt, is the synthetic form ...
Etomidate, a highly selective intravenous anesthetic agent, was first synthesized at Janssen Pharmaceuticals in ...
Muramyl dipeptide (MDP) is the smallest structural unit with immune activity in the skeleton of bacille calmette ...
Teriparatide is a human parathyroid hormone analog with the same structure as the N-terminal 34 amino acid seque ...