Surface Plasmon Resonance imaging (SPRi), namely surface plasmon resonance microscopy (SPRM) is an optical technique that is used to spatially monitor localized differences in the reflectivity of incident light from a prism-gold film interface that result from molecules adsorbing to or desorbing from the gold film. It can realize label-free, real-time, highly sensitive, quantitative, high-throughput biological interaction monitoring and the binding profiles from multi-analytes further provide the binding kinetic parameters between different biomolecules. What's more, SPR imaging can be used to directly study the interactions of the carbohydrate arrays with proteins adsorbing from solution without the use of a reporter group.
In addition to the benefits afforded from the increased functional affinity and specificity of a surface-based assay format, the use of SPR to monitor protein-carbohydrate interactions with carbohydrate arrays has distinct advantages over fluorescence methods. Specifically, lower affinity interactions can be detected because SPR measurements can be made in the presence of a large excess of unbound protein, without the background problems that would complicate a similar measurement using fluorescence based techniques.
Fig1. Fabrication of carbohydrate-conjugated SPRi sensors (A. Tyagi et al. 2010)
Cell recognition is based on protein-carbohydrate interactions such as cell adhesion, transport, apoptosis, and immune responses. Since lectins have a carbohydrate-binding domain, these interactions can be shown on cell surfaces and lectins by glycoproteins, glycolipids and polysaccharides, but these interactions are usually weak. So SPRi technology provides a good tool for protein-carbohydrate interactions research, and it is essential for understanding biological functions and the role these interactions play in disease states.
Creative Peptides provides professional SPR analysis services, we can follow the customer's request for high-precision analysis of protein/peptide-carbohydrate interactions while ensuring reasonable price and accurate analytical data.
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Protein-carbohydrate interactions are crucial in various biological processes like cell recognition, immune responses, and transport. These interactions occur when proteins bind to carbohydrates, such as glycoproteins, glycolipids, and polysaccharides, often at cell surfaces.
Surface Plasmon Resonance imaging (SPRi) is a powerful optical technique used to study protein-carbohydrate interactions in real-time. It provides label-free, high-sensitivity measurements of binding kinetics, allowing for detailed analysis of low-affinity interactions that are difficult to detect with fluorescence methods.
SPRi allows for highly sensitive, quantitative, and high-throughput analysis of protein-carbohydrate interactions without the need for labeling. It can detect weak interactions and provides clear, reproducible results even in the presence of unbound proteins.
SPRi is essential for studying biological functions like cell adhesion, transport, apoptosis, and immune responses, as well as understanding the role of these interactions in diseases. It is widely used in glycobiology and the development of therapeutics targeting carbohydrate-binding proteins.
Creative Peptides offers high-precision SPR analysis for protein/peptide-carbohydrate interactions. With advanced equipment, an experienced scientific team, and competitive pricing, we provide reliable and reproducible results tailored to customer needs.
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