Custom Labeling

Peptides can be labeled by different methods depending on their amino acid composition, and a variety of labeling modes can be selected. The peptide labeling belongs to a kind of peptide modification synthesis, and the addition of appropriate label in the peptide molecule is helpful for the study of the peptide. Creative peptides offers peptide synthesis with different labels matching various research issues.

Our peptide labeling services:

1. Stable isotope labeled peptides

Isotopic labeling refers to the replacement of atoms with different heavy isotopes. A common alternative are ¹²C by ¹³C, of ¹⁴N by ¹⁵N, and of ¹H by ²H (deuterium). Creative Peptides can provide customized peptides labeled with stable isotopes including ²H, ¹⁵N & ¹³C, respectively, or a combination of ¹⁵N & ¹³C. Even when the peptides are attached with specific modification motifs.

1. Photo cross linkers

Studies of peptide-protein or protein-protein interactions often involve the use of cross-linking agents, of which photo cross linkers are an effective tool. Creative Peptide can connect appropriate photo cross linkers to peptide, and commonly used photo cross linkers are Bpa and DMNB.

1. Spin labels

Spin labeling is also a good label pattern in studying peptide-protein and protein-protein interactions. The combination of site-directed spin labeling (SDSL) technology and electron paramagnetic spectroscopy (EPR) is a mature method that is widely used in protein and peptide science. Creative Peptides can apply this technique to the research of interaction of peptides with biofilms, as well as the secondary structure, position and orientation of peptides in membrane systems. TEMPYO and TOAC are available spin labels.

1. Linkers & Spacers    

A linker, also called spacers, refers to an extension of a bendable molecule or molecule that can be used to join two molecules of interest, such as a fluorophore attached to one or two peptide molecules. Depending on the design and application, Creative Peptides can insert the appropriate linker between the tags, proteins or fluorophores and peptides. Including but not limited to the following services.

References:
[1] Yan, F., Che, F. Y., Nieves, E., Weiss, L. M., Angeletti, R. H., & Fiser, A. (2011). Photo‐assisted peptide enrichment in protein complex cross‐linking analysis of a model homodimeric protein using mass spectrometry. Proteomics, 11(20), 4109-4115.
[2] Smirnova, T. I., & Smirnov, A. I. (2015). Peptide–Membrane Interactions by Spin-Labeling EPR. In Methods in enzymology (Vol. 564, pp. 219-258). Academic Press.