Acetylation refers to the substitution of hydrogen by acetyl group in organic molecules with oxygen, nitrogen, carbon, sulfur atoms. Commonly used acylation agents are carboxylic acylation agents, carboxylic ester acylation agents, anhydride acylation agents and acyl chloride acylating agents. Acyl groups are the groups remaining after removal of hydroxyl groups from organic acids, inorganic acids, sulfonic acids, etc. Chemically synthesized peptides often carry free amino groups and free carboxyl groups. The sequence of peptide often represents the sequence of female protein.
In order to be closer to female protein, the end of peptide often needs to be closed, that is, N-terminal acetylation and C-terminal amidation, which can reduce the total charge of peptide and the solubility of peptide. Peptides can also mimic its original state of α-amino and carboxyl groups in maternal proteins. Acetylation modification of peptides is a reversible post-translational modification process that can be expressed in both eukaryotic and prokaryotic cells. In the past few decades, many researches have been carried out on the acetylation modification of amino acids.
Creative Peptides offers custom production of peptides containing acetylated residues. The incorporation of acetylated Lysine residues is in most cases straight forward. Nevertheless, when the objective is to obtain high quality material, we know from experience that you need the better reagents, a sound synthesis methodology, and quality control to match it.
We offer excellent acetylated peptides that:
Every step of peptide synthesis is subject to Creative Peptides’ stringent quality control. Typical delivery specifications include:
Peptide acetylation is the process of attaching an acetyl group (-COCH3) to the amino group at the N-terminus of a peptide. This modification reduces the overall charge of the peptide and mimics the structure of natural proteins, improving its solubility and stability. Acetylation also helps prevent degradation at the N-terminal, which is crucial for enhancing peptide longevity in biological applications.
Acetylation removes the positive charge from the N-terminal amino group, enhancing the solubility and stability of peptides. It mimics the structure of natural proteins, allowing peptides to interact more efficiently with cellular environments. Additionally, acetylation can prevent the N-terminal degradation of peptides, improving their stability during research or experimental procedures.
Yes, acetylated peptides can be expressed in both eukaryotic and prokaryotic systems. The acetylation process is reversible and can be adapted for use in various biological expression systems, making it versatile for a wide range of research applications in different organisms.
Acetylating peptides provides several benefits, such as mimicking the structure of natural proteins by modifying the N-terminus. This modification improves the solubility and stability of peptides, while also preventing N-terminal degradation. Acetylation enhances the overall functionality of peptides, making them ideal for studies on protein stability, interactions, and other biochemical assays.
Acetylated peptides are synthesized using advanced peptide synthesis techniques, ensuring high-quality production. After synthesis, the peptides undergo strict quality control processes, including mass spectrometry to confirm the acetylation site and verify the number of modified residues. The peptides are also analyzed using HPLC to assess purity, and a synthesis report is provided to confirm quality.
Yes, acetylation can be applied to peptides with various amino acid sequences, particularly those containing lysine residues. The process can be tailored to target the N-terminal amino group or lysine residues, allowing for customized peptide modifications depending on the specific research needs and peptide sequence.
Acetylated peptides are commonly used in structural studies, enzyme activity assays, and protein interaction research. They are particularly useful in biological assays that require stable, soluble peptides, such as those focused on cellular uptake, receptor binding, and other cellular functions.
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