NHS-Activated Peptide Conjugation Magnetic Beads, also called immunomagnetic microspheres, is a small spherical particle with superparamagnetic and protective shell, which is basically composed of carrier microspheres and immune ligands. Its core is paramagnetic particle, core outer layer is encapsulates polymer, the outermost layer is immune ligand. NHS-Activated Peptide Conjugation Magnetic Beads technique is an immunological detection and isolation technique based on specific antigen-antibody reaction. The antigen-antibody complex is formed by binding the antibody with the specific antigen in the reaction medium by using the magnetic beads coated with the antibody as the carrier. The complex moves in a directional direction under the action of the external magnetic field, thus achieving the purpose of isolating the antigen.
NHS-Activated Peptide Conjugation Magnetic Beads is mainly used in the field of separation and purification, such as protein separation and purification, antibody separation and purification. Compared with traditional carboxyl and amino beads, NHS beads need not be activated by EDC or glutaraldehyde in advance. The two main types of NHS-Activated Peptide Conjugation Magnetic Beads on the market are Mag NHS and Magrose NHS.
Mag NHS is a silica coated magnetic beads with a particle size of 1-2 μm. It is mainly used in the field of analysis and detection, such as in vitro diagnosis, microbial detection, immunoprecipitation, co-immunoprecipitation and so on.
Magrose NHS is a porous microsphere based on natural hydrophilic polymer agarose, with a diameter of 30 μm. It is mainly used in the field of separation and purification, such as protein separation and purification, antibody separation and purification.
Creative Peptides provides uniform, silicon-based superparamagnetic particles coated with a high density NHS (N-hydroxysuccinimide) functional group. These beads are capable of specifically binding to a primary ligand containing an amine. It has the following characteristics:
Creative Peptides has developed a reliable method for synthesizing NHS-Activated Peptide Conjugation Magnetic Beads, and the company will provide NHS-Activated Peptide Conjugation Magnetic Beads to global customers with excellent technology. The experienced experts and advanced instruments in the Creative Peptides can meet all your needs for peptide-NHS magnetic bead coupling. We will provide you with a full range of synthesis and modification services.
NHS-Activated Peptide Conjugation Magnetic Beads are primarily used for protein separation, purification, and antibody isolation. These beads are coated with NHS functional groups that allow for the specific binding of peptides or proteins with amine-containing ligands, enabling their isolation under an external magnetic field. This makes them ideal for immunoprecipitation, co-immunoprecipitation, and other analytical applications.
NHS-Activated Magnetic Beads work by forming a covalent bond with peptides or proteins containing amine groups. The NHS (N-hydroxysuccinimide) group on the beads reacts with the amine group of the peptide, forming a stable conjugate. Once conjugated, these magnetic beads can be used to isolate and purify the peptide of interest using a magnetic field, ensuring high specificity and low non-specific binding.
Mag NHS beads are silica-coated magnetic particles, typically used for analysis and detection, including in vitro diagnostics and microbial detection. In contrast, Magrose NHS beads are agarose-based porous microspheres, commonly used for protein separation, purification, and antibody isolation due to their larger surface area for conjugation.
Yes, NHS-Activated Magnetic Beads are reusable. After use, the peptide-conjugated beads can be regenerated and reused for multiple rounds of isolation or purification, making them a cost-effective solution for high-throughput applications. However, proper handling and cleaning protocols should be followed to maintain their binding efficiency.
Choosing the right NHS-Activated Magnetic Bead depends on your specific application needs. For detection-based applications or smaller scale analysis, Mag NHS with its smaller particle size is a suitable option. For larger-scale separation and purification tasks, such as protein or antibody isolation, Magrose NHS with its larger surface area and higher capacity may be more appropriate. Consider factors like bead size, surface area, and the type of ligands being conjugated.
The advantages of using NHS-Activated Peptide Conjugation Magnetic Beads include high capacity for binding, low non-specific binding, and stable covalent bonding that minimizes ligand leakage. They are pre-activated and ready-to-use, making them highly efficient for rapid conjugation and purification. Additionally, they produce reusable immunoaffinity matrices, which enhance their utility in various applications.
NHS-Activated Magnetic Beads improve peptide purification by enabling selective, covalent attachment of peptides to the beads via amine groups. This specificity ensures that only the target peptide is captured, reducing the risk of contaminant binding. The magnetic beads can then be easily separated using a magnetic field, making peptide isolation and purification faster and more efficient compared to traditional methods.
References
