Order Antibody Blocking Peptides | Custom Synthesis

Designed for biological research and industrial applications, not intended for individual clinical or medical purposes.

Product Name	Appearance	Source	Storage Conditions	Shipping Conditions
CD40 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
IFN beta Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
OR5F1 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
HMGB2 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
SATB1 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
SLC2A3 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20℃，2 years.(Avoid freeze/thaw cycles)	Shipped on dry ice
VAMP1 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
CDK5 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
NUS1 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
MYLK Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
POPD1 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
PCYT2 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
CCL4L1/CCL4 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
ZCCHC4 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20℃，2 years.Avoid freeze/thaw cycles.	Shipped on dry ice
TERT Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
ADGRG6 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
Acvrl1 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
CENPA Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
TLR7 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice
BMP6 Antibody Blocking Peptide	Lyophilized powder	Synthetic	Store at -20°C. Avoid freeze / thaw cycles.	Shipped on dry ice

Antibody blocking peptides are short, sequence-defined synthetic peptides that correspond precisely to the immunogen region used to generate a specific antibody. By competitively binding to the antibody’s paratope, these peptides serve as a gold-standard control for confirming antibody specificity across multiple immunodetection platforms, including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA. When a blocking peptide effectively abolishes or significantly reduces the antibody signal, researchers gain strong evidence that the antibody is recognizing its intended epitope rather than nonspecific targets. Our blocking peptides are manufactured with ≥95% purity and undergo rigorous analytical verification to ensure consistency, bioactivity, and compatibility with assay conditions. Designed to support both routine laboratory validation and high-stakes research applications, these peptides provide a reliable and reproducible strategy for minimizing off-target binding, reducing background noise, and enhancing the interpretability of antibody-based experiments. Custom peptide synthesis services are also available for laboratories requiring tailored immunogen sequences, specialized modifications, or scaled production.

How Blocking Peptides Work

Blocking peptides function through competitive inhibition, a mechanism in which a synthetic peptide—identical or highly homologous to the antibody’s immunogen sequence—occupies the antibody’s antigen-binding site (paratope) before the antibody encounters its target protein. During pre-incubation, the blocking peptide binds to the antibody via sequence-specific interactions, effectively saturating the recognition sites that would otherwise engage with the corresponding epitope on the sample.

When the peptide-antibody mixture is applied to a Western blot membrane, tissue section, or cultured cells, the saturated antibody is no longer able to bind to the endogenous protein. As a result, a significant reduction or complete loss of signal is observed. This differential signal—with vs. without blocking peptide—serves as a powerful internal control to validate antibody specificity, rule out cross-reactivity, and distinguish true target detection from off-target or non-specific binding.

Because of this mechanism, blocking peptides are widely used in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA, where antibody specificity is critical for accurate interpretation. The approach is especially valuable for antibodies targeting protein families with conserved domains, low-abundance targets, or membrane proteins prone to cross-reactivity. By providing a direct epitope-level competition, blocking peptides enable researchers to confirm true antigen recognition, enhance confidence in experimental results, and improve reproducibility across assays.

Custom Blocking Peptide Services

We offer a full suite of customizable peptide solutions designed to support precise antibody validation, assay optimization, and advanced research workflows. Each service is executed with strict quality control and delivered with complete analytical documentation.

Immunogen Sequence Design

Epitope Selection—Bioinformatic analysis to identify the exact sequence used for antibody generation.
Specificity Optimization—Ensures minimal homology to unrelated proteins to avoid off-target effects.
Design Validation—Computational review of peptide properties, immunogenic regions, and structural exposure.

Precision Peptide Synthesis

High-Fidelity Solid-Phase Synthesis—Accurate amino acid incorporation to ensure sequence integrity.
Purity Optimization—Standard ≥95% purity with higher grades available upon request.
Batch Consistency—Reproducible synthesis protocols for reliable performance across experiments.

Peptide Cyclization Options

Head-to-Tail Cyclization—Enhances structural rigidity and improves stability in biological environments.
Side-Chain Cyclization—Ideal for mimicking conformational epitopes or increasing binding affinity.
Stability Enhancement—Reduces degradation and boosts performance in repeated or long-term assays.

Advanced Peptide Modifications

Functional Labeling—Biotin, FITC, or other tags for detection and pull-down applications.
Post-Translational Modifications—Phosphorylation, acetylation, methylation, or custom PTMs.
Terminal Protection—N-terminal acetylation or C-terminal amidation to enhance stability and solubility.

Flexible Production Scaling

Research-Scale Synthesis—Milligram-level production for small projects and pilot studies.
Bulk Manufacturing—Gram-scale batches suitable for commercial or long-term research needs.
Rapid Turnaround—Optimized workflows for fast delivery without compromising quality.

Rigorous Quality Control

HPLC Purity Analysis—Complete chromatographic profiling for each batch.
Mass Spectrometry Confirmation—Validates molecular weight and structural identity.
Documentation Package—Full analytical report included for publication or regulatory requirements.

Confidentiality & IP Protection

Secure Sequence Handling—All peptide sequences are treated with strict confidentiality.
Protected Data Storage—Encrypted systems ensure safe management of customer information.
Non-Disclosure Support—NDA agreements available for sensitive research projects.

Expert Technical Consultation

Sequence Review & Optimization—Professional guidance to refine peptide design for your assay.
Protocol Support—Recommendations for peptide-to-antibody ratios and blocking conditions.
Troubleshooting Assistance—Direct consultation for improving assay performance or resolving signal issues.

Key Features & Benefits

Immunogen-Matched Sequence — Each blocking peptide is precisely designed to match the antibody’s immunogen region, ensuring accurate specificity verification.
High Purity (≥95%) — HPLC-purified peptides deliver reliable competitive binding and minimal background in immunoassays.
Antibody Specificity Validation — Provides a gold-standard control to confirm target recognition in WB, IHC, IF, and ELISA.
Reduces Non-Specific Binding — Effectively blocks off-target interactions to improve signal clarity and experimental reproducibility.
Ready-to-Use or Customizable — Available as catalog peptides or fully customized to your required sequence and modifications.
Compatible With Multiple Applications — Optimized for Western blot, immunofluorescence, immunohistochemistry, and other antibody-based assays.
Rigorous QC & Analytical Validation — Verified by mass spectrometry and HPLC to ensure sequence integrity and batch consistency.
Flexible Production Scale — Small-quantity research batches and large-scale manufacturing available on request.

Applications of Antibody Blocking Peptides

Blocking peptides play a critical role in confirming antibody specificity and improving the reliability of immunodetection assays. They are widely used across multiple research platforms where accurate target recognition is essential.

Western Blot (WB) Validation

Signal Verification—Confirms that detected bands correspond to the intended target protein.
Cross-Reactivity Detection—Helps identify non-specific or off-target bands in complex lysates.
Quantitative Confidence—Enhances interpretation of band intensity differences across samples.

Immunohistochemistry (IHC)

Specificity Control—Ensures staining patterns reflect true protein localization in tissue sections.
Background Reduction—Minimizes false-positive staining caused by non-specific antibody binding.
Morphological Accuracy—Helps distinguish target-driven signal from tissue artifact staining.

Immunofluorescence (IF) & Confocal Imaging

Fluorescence Signal Validation—Confirms that fluorescence originates from true antigen–antibody interaction.
Artifact Elimination—Reduces auto-fluorescence and antibody-independent signal noise.
Co-Localization Reliability—Strengthens confidence in multi-channel imaging and co-expression studies.

ELISA & Peptide-Based Assays

Epitope Specificity Check—Verifies that antibody signal corresponds to the correct binding epitope.
Competitive Inhibition Studies—Supports binding competition assays and interaction mapping.
Assay Optimization—Improves reproducibility by validating antibody performance under assay conditions.

Cross-Reactivity Assessment

Protein Family Differentiatio—Confirms selective recognition among homologous or conserved-domain proteins.
Off-Target Identification—Helps detect unintended antibody binding in multiplex experiments.
Reagent Qualification—Supports assay development and quality control for diagnostic workflows.

Novel Antibody Development

Epitope Confirmation—Confirms that newly generated antibodies recognize the expected target region.
Clone Selection Support—Helps screen monoclonal antibody candidates for specificity.
Preclinical Research Compatibility—Useful in validating antibodies used in biomarker discovery and mechanism studies.

FAQs

1. Can blocking peptides be used as positive controls?

No. They are used as specificity controls to block antibody signal.

2. How much peptide should I use?

Blocking peptides are typically used at a 5–20× molar excess relative to the antibody.

Order Antibody Blocking Peptides or Request Custom Synthesis

Elevate the accuracy and reliability of your antibody-based experiments with our high-quality blocking peptides. Whether you need a catalog sequence or a fully customized design, our specialists are ready to support your research with precision synthesis, rigorous quality control, and rapid turnaround. Contact us today to request a quote, discuss your project needs, or start designing your custom blocking peptide solution.