Leveraging our expertise and knowledge in peptides manufacturing, we design and synthesize a variety of combinatorial peptides to meet the needs of customers by combining combinatorial chemistry and computer chemistry.
In the past decade, with the infiltration of computer technology, new material technology, molecular biology and immunohistochemical technology into pharmaceutical chemistry, and the rapid development of peptide synthesis technology in practice, it is possible to establish modern synthesis methods to meet the assumption of rapid and accurate search for lead compounds in new drug development. Combinatorial peptide synthesis chemistry based on solid phase peptide synthesis provides a new idea for rapid and massive synthesis of bioactive peptides, and has become a hot spot in the research and development of new peptide drug.
Polyethylene needle-shaped rods are solid phase carriers, which are first connected to one end of the rods and fixed on the other end of the rods. Each board can fix dozens of parallel rods, pay attention to regular arrangement, and the distance between the two needles is appropriate. During the reaction, the small rod head of the plate was immersed in the corresponding titer plate titer tank for condensation reaction, the whole process was completed by Merrifield standard synthesis method, and finally the protective group was removed, but the peptide chain was not cut off from the carrier, and the peptide was suspended on the resin for many times to study its activity.
The solid phase carrier was filled with polyethylene or polypropylene mesh with micropore (74μm) as a container. During the reaction, several small bags were immersed in the solvent of the same reactor to carry out the solid phase peptide grafting reaction of deprotection, washing and condensation cycle. If the next amino acid is different, some small bags can be taken out and put into another reactor for the next peptide reaction. Finally, taking each small bag as a combination, the free related peptides are cleaved and the biological activity is screened.
The cellulose material is used as the solid phase carrier, and the carrier disk is loaded into the glass column after the peptide structure is attached to the carrier surface-OH. The more carrier slices per column, the greater the synthesis quantity. The columns are arranged, and each column synthesizes a peptide analogue. The more the column, the more kinds of products. When shrinking to different components, each column reacts separately. After synthesis, the protective groups were removed and then split separately to get the prepared products. For rapid coarse screening, array synthesis on fiber paper is developed, in which the prefabricated activated carboxyl components are added to the "amino point" in the interval 1cm, and a few hours later, the peptide cycle is repeated according to washing-depreservation-washing-condensation and so on.
After the glass carrier was treated, the surface of the glass carrier was aminated, and then the surface of the photosensitive N-terminal protective group was combined with NH2 as the N-terminal protective group of its amino acid. The deprotected Novc region was selected by light irradiation, and then carboxyl components were added for peptide condensation reaction. The irradiation area was divided by orthogonal single illumination method and binary semi-illumination method. After the reaction, the N-terminal photosensitive protection group and side chain protection group were removed, and the slide was immersed in the fluorescence labeled receptor solution and scanned under the fluorescence microscope, and the active peptide region was determined according to the fluorescence of the specific peptide-receptor affinity region. in order to complete the whole light-controlled combinatorial chemistry method.
Each time an amino acid is connected, the "uniform mixing" equal fraction is repeatedly condensed with the carboxyl component with activated ester, and at the beginning of each cycle, the reactants in the reactor are "statistically pure" until they are completed. Because the coupling is placed on the reaction beads, the reaction beads with different peptide pieces come into contact with only one amino acid in the same reaction container, which avoids the competition between slow and fast coupling and greatly increases the number of synthetic peptides.
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