Stable Isotope Labeled Peptides

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).

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Creative Peptides is equipped with a strong team of technical personnel and advanced equipment. We have rich experience in research and development and production of new isotope compounds, and constantly breakthrough and innovate to synthesize different types of stable isotopes according to the requirements of our customers, and we always guarantee high quality and efficient service. Creative Peptides can customize peptide products containing different stable isotope atoms according to customers' requirements, and deliver them quickly after completion on time.

What are Stable Isotope Labeled Peptides?

Stable isotope labeled peptides are chemically synthesized peptides with natural sequences. In stable isotope labeled peptides, one or more atoms in the peptide chain are replaced with the corresponding stable isotope, such as deuterium (2H), carbon-13 (13C), or nitrogen-15 (15N), instead of its common natural isotope. Stable isotope labeled peptides are chemically similar to unlabeled peptides. Labeled and unlabeled peptides exhibit similar behavior in terms of reactivity, solubility, and adsorption.

Overview of quantification approaches in LC-MS based proteomic experiments.Fig. 1 Overview of quantification approaches in LC-MS based proteomic experiments. [4]

Synthesis Steps of Stable Isotope Labeled Peptides

At Creative Peptides, we can customize the synthesis of many types of peptides. Based on our specialized capabilities and technologies, we can respond proactively to the changing needs of high-quality stable isotope labeled compounds. Upon completion of the custom synthesis, all stable isotope labeled peptides are subjected to rigorous mass spectrometry and HPLC analysis to determine final purity and ensure that our clients receive only the highest quality peptides. Creative Peptides is a reliable partner to work with to accelerate the pace of achieving your research goals.

  • Peptide sequence design: This process involves obtaining the customer's requirements and evaluating and optimizing the designed sequence.
  • Isotope labeling selection: Selection is made based on the common stable isotope options below and the feasibility of the solution is evaluated.
  • Stable isotope peptides synthesis: After completing the above steps, peptides can be prepared using our specialized isotope labeling techniques. Isotope labeling techniques include, deuterium labeling, carbon labeling, nitrogen labeling and oxygen labeling.
  • Labeling efficiency and quality control: Labeling efficiency is the ratio of stable isotopes of the labeled peptide, ensuring high efficiency and high purity of labeling can improve the accuracy and reliability of the experimental results. And after the completion of stable isotope labeling of peptides, quality control is needed to ensure the purity and correct labeling.

Stable Isotope Options

  • 2H
  • 13C
  • 15N
  • 15N and 13C combinations
  • 18O

Data Interpretation and Analysis

Creative Peptides provides isotope ratio calculations, metabolic kinetic studies, and pharmacokinetic analyses when using stable isotope-labeled peptides for experiments and analyses to ensure proper interpretation of data and appropriate statistical analysis. Finally, Creative Peptides will deliver the synthesized stable isotope-labeled peptides with associated reports, including purity, quality analysis results, and confirmation of isotope labeling.

  • Stable isotope labeled peptide of specified quantity and purity
  • HPLC chromatogram
  • MS spectra
  • Certificate of analysis

Our Service Features

Creative Peptides provides stable isotope labeled custom peptides for determining protein structure and dynamics or protein-protein interactions because it allows for the doping of NMR active nuclei, which helps to reduce the complexity of spectroscopy and helps researchers to obtain correlations between atoms for more complete structural information.

  • Design and synthesize peptides with expected mass differences
  • Tracking peptide metabolic pathways and kinetics
  • Quantitative analysis, especially in metabolic studies and protein quantification
  • Various modifications possible, e.g. disulfide bond formation
  • Tracking of intracellular location and migration of proteins
  • Cost-effective analytical services

References

  1. Becker GW. (2008). Stable isotopic labeling of proteins for quantitative proteomic applications. Briefings in Functional Genomics and Proteomics, 7(5), 371-382.
  2. Kettenbach AN, et al. (2011). Absolute quantification of protein and post-translational modification abundance with stable isotope–labeled synthetic peptides. Nature protocols, 6(2), 175.
  3. Ong SE, et al. (2002). Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Molecular & cellular proteomics, 1(5), 376-386.
  4. Mueller LN, et al. (2008). An assessment of software solutions for the analysis of mass spectrometry based quantitative proteomics data. Journal of proteome research, 7(01), 51-61.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
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