Stable Isotope Labeled Peptides

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).

Online Inquiry

Stable isotope labeled peptides (SIL peptides), also known as heavy peptides, are chemically synthesized peptides with the native sequence, but some of the constituent amino acids are replaced by the stable isotope labeled amino acids, such as 12C by 13C, 14N by 15N, and 1H by 2H. Stable isotope labeled peptides are chemically and physically indistinguishable from their endogenous counterparts with respect to retention time, ionization efficiency and fragmentation mechanism. They only differ by mass. These properties make the stable isotope labeled peptides could be widely applied in quantification analysis (e.g. ICAT, iTRAQ and AQUA), the nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) and reference materials for pharmacokinetic analyses and metabolite identification. Other applications include the protein structure analysis, protein expression monitoring, protein cross-linking analysis, quantitative proteomics, biomarker discovery, cell signal profiling, and pathway validation.

Overview of quantification approaches in LC-MS based proteomic experiments.Fig.1 Overview of quantification approaches in LC-MS based proteomic experiments. [4]

As a world-leading peptide manufacturing organization, Creative Peptides possesses extensive expertise and years of experience which is able to provide customized stable isotope labeled peptides synthesizing service to customer worldwide. All stable isotope labeled peptides are synthesized using the latest Fmoc solid-phase peptide technology and performed mass spectrometric analysis and stringent analytical HPLC to establish the final purity, to assure that our customers receive the highest quality peptides. Creative Peptides is a reliable partner that can work with you to accelerate the speed to achieve your research goals.

Stable Isotope Options

Service Features

References

  1. Becker GW. (2008). Stable isotopic labeling of proteins for quantitative proteomic applications. Briefings in Functional Genomics and Proteomics, 7(5), 371-382.
  2. Kettenbach AN, et al. (2011). Absolute quantification of protein and post-translational modification abundance with stable isotope–labeled synthetic peptides.Nature protocols, 6(2), 175.
  3. Ong SE, et al. (2002). Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Molecular & cellular proteomics, 1(5), 376-386.
  4. Mueller LN, et al. (2008). An assessment of software solutions for the analysis of mass spectrometry based quantitative proteomics data.Journal of proteome research, 7(01), 51-61.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.

Customer Support & Price Inquiry

Resource

Creative Peptides is a custom lab chemical supplier that provides a wide range of peptides for the pharmaceutical and biotechnology industries.

Learn More

Function of M871 as a Galanin GAL2 Receptor Antagonist

 Galanin-(2–13)-Glu-His-(Pro)3-(Ala-Leu)2-Ala-amide (M871) is a novel peptide antagonist selectively recognizing ...

Glucagon and Glucagon signaling

Glucagon is a 29-amino acid peptide hormone that is synthesized in pancreatic α cells from the proglucagon precursor by proho ...

What Is Anisomycin?

Anisomycin, also known by its trade name flagecidin, is a bacterial pyrrolidine antibiotic mostly isolated from ...

Function of Kassinin in Stimulating Ion Transport in Skin

 Kassinin, a new peptide of amphibian origin, has been traced in the skin of the African frog Kassina senegalensi ...

Function of pergolide mesylate salt in Parkinson's disease

  Pergolide mesylate salt , also known as 8-beta-((methylthio)methyl)-D-6-propylergoline methanesulfonate, is a ...

Contact Us

Address:

Tel: |

Email:

Copyright © 2022 Creative Peptides. All rights reserved.