Targeting Peptide Modification and Conjugation

N/C-terminal modifications

N-terminal modifications: Acetylation, Biotinylation, Fluorescent labeling (FITC, Cy5, TAMRA, etc.), Radioactive chelators (e.g., DOTA, NOTA)

C-terminal modifications: Amidation, Fatty acid modifications (e.g., palmitoylation), Polyethylene glycol (PEGylation)

Biotinylation

FITC / Rhodamine labeling (fluorescent tags)

PEGylation (to improve stability and in vivo circulation time)

Amino Acid Site-specific Modifications

Hydroxyl, amine, thiol site modifications

D-amino acid substitutions (to enhance protease stability)

Phosphorylation, methylation, sulfation modifications

Introduction of bifunctional sites (e.g., simultaneous incorporation of fluorescent and linker groups)

Incorporation of Non-natural Amino Acids

D-amino acids (for enhanced stability)

Non-natural amino acids (e.g., Azido-Lysine, Alkyne-Lysine, facilitating click chemistry)

Amino acids containing reactive groups (e.g., azide, alkyne, maleimide, carboxyl groups)

Alkyl chain elongation or branched amino acid introduction

Directed design of conjugation sites

Chemical Modification and Protection

Customized side-chain protection/deprotection

Photosensitive/pH-sensitive linker design

Multiple modifications (e.g., simultaneous PEGylation and fluorescent labeling)

Peptide-Drug Conjugates (PDC)

Conjugation with antitumor drugs (e.g., doxorubicin, paclitaxel, camptothecin)

Conjugation with toxin molecules (e.g., MMAE)

Conjugation with hydrophobic small molecules (to enhance membrane permeability or target binding)

Protein/Antibody Conjugation

Peptide-antibody conjugation (for ADC precursors or immune recognition)

Conjugation with enzymes, hormones, cytokines, and other protein molecules

Cross-species protein linker design (for functional studies)

Nanocarrier/Surface Conjugation

Targeting peptides modification on liposomes, polymer nanoparticles, gold nanoparticles surfaces

Peptide conjugation with microspheres, magnetic beads, scaffold materials (for in vitro targeted enrichment)

Construction of delivery systems targeting RNA, DNA (e.g., conjugation with siRNA, ASO nucleic acids)

Imaging Probe Conjugation

Fluorescent probes (FITC, Cy3, Cy5, IRDye)

MRI contrast agents (Gd-DOTA, etc.)

Radiopharmaceutical chelators (DOTA, NOTA) conjugated with peptides

Click Chemistry Conjugation Services

CuAAC (copper-catalyzed azide-alkyne cycloaddition)

SPAAC (strain-promoted azide-alkyne cycloaddition)

Applicable for constructing multivalent, multifunctional targeting systems

Crosslinker Conjugation Services

Use of crosslinkers such as Sulfo-SMCC, EMCS, DSP, MBS for heterobifunctional conjugation

Achieving peptide-protein, peptide-polypeptide, peptide-polymer structural linkages

Conjugation strategy design and site screening: Providing structural analysis and molecular docking suggestions to select optimal modification/conjugation sites while avoiding loss of target activity

Purity analysis and structural characterization: Offering multiple analytical methods including HPLC, LC-MS, MALDI-TOF, NMR, SEC-MALS to confirm product structure

Scale-up production support: From milligram-scale R&D to gram-scale pilot production, capable of GLP or GMP-level projects

Customized reports: Providing detailed records of modification and conjugation processes, structural confirmation data, conjugation efficiency, and functional testing data (e.g., binding assays)