The analysis and identification of peptides is one of the most basic techniques in peptide research and the key to determining peptide components. According to the basic properties of the peptide, it is separated and purified, finally analyzed and identified by pure product. If the characterization of the polypeptide is not well understood, subsequent studies will be difficult. Therefore, we will provide a series of the best quality services for the analysis of peptide characterization.
Characterization of the structure of a peptide is critical to understanding the function of the protein. Our characterization of peptide services provides deep, highly sensitive assays for the purification of derived peptide samples or expression of peptides samples.
Fig. 1 Analysis of peptide characterization (Addonizio, 2017)
Creative Peptides has established a series of peptide N-terminal, C-terminal analysis test platforms, which have strong sensitivity and reproducibility and have been well applied at the protein level. By tandem mass spectrometry, the polypeptide chain regular broken, fragments of peptides obtained, The N-terminal, C-terminal sequence can be obtained from spectrum analysis. In addition, we established an automated sequence analyzer for sequencing based on the principle of Edman's degradation method. In the sequence analysis, the sequence of the amino acid sequence of the target product is compared with the sequence deduced by the gene sequence, thereby obtaining the sequence information of the peptide more accurately.
Creative Peptides provides the highest resolution of the crystal structure analysis of peptides. Among them, the most important links are: data processing and analysis, heavy atom positioning, density modification, molecular replacement, graphic integration, model processing and validation. A range of services created by us can reduce the conditions for groping peptide crystals, quickly analysis the crystal structure data, and reduce analytical errors. Finally, we will give you a detailed report analysis.
Comprehensive peptide characterization services include quality identification (molecular weight), charge identification (isoelectric point), structural identification (primary, secondary, and tertiary structures), and biological activity analysis. Additional services include immunological identification and specific enzymatic kinetics testing.
Peptide characterization is essential for ensuring the correct molecular structure, activity, and functionality of peptides. This ensures that the peptides are suitable for subsequent applications in drug development, diagnostics, and therapeutic research.
Mass spectrometry (MS) is used to accurately identify the molecular weight of peptides, confirming their purity and identity. This method is essential for verifying the peptide's integrity and suitability for research.
Isoelectric focusing and capillary electrophoresis are commonly used to determine the isoelectric point (pI) of peptides. Understanding the pI is important for determining the peptide's charge properties and its behavior in biological systems.
Yes, peptide 3D structure analysis is performed using high-resolution crystal structure techniques such as X-ray diffraction. This analysis provides detailed insights into the peptide's conformation, which is critical for understanding its functional properties.
Tandem mass spectrometry and automated sequence analyzers using Edman's degradation method are employed to accurately determine the N-terminal and C-terminal sequences. These analyses ensure the peptide's integrity and correct sequence for various applications.
Yes, biological activity testing is provided, including assessments of enzyme kinetics, receptor binding, and cell-based assays. These tests help evaluate the peptide's effectiveness and suitability for therapeutic or diagnostic applications.
Immunological identification involves techniques such as antigen-antibody reactions and enzyme-linked immunosorbent assays (ELISA). These methods assess the peptide's potential for immunogenic responses and its suitability for vaccine or antibody development.
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