Interested in the use of biologically active proteins and peptides as potential therapeutic agents has grown dramatically in recent years. Although many meaningful studies can be performed, it is important to consider several factors such as the length of sequence, amino acid residues and the sequence of peptides, all of which will influence whether correct assembly and purification are feasible. If you would like Creative Peptides to help you in designing your peptide, our technical support department can assist you.
The following suggestions should be considered in the design of a peptide. They also determine the solubility of the final product.
The full de novo peptide design framework is described in detail in the part of de novo peptide design. It consists of three stages: an optimization-based sequence selection, fold specificity calculation, and approximate binding affinity calculation.
Our technical and applications support team can offer advice on library design and experimental set up and analysis. Relating to design, we can advise on the best overlap or offset of peptides to be used in your target application, and then using your full-length protein sequence, we can generate the list of peptides for your library.
Stapling is a key technique for stabilizing peptides in an α-helical structure. The resultant stapled peptides are then able to compete efficiently for binding to protein targets involved in protein-protein interactions that are mediated by α-helices.
Cell-Penetrating Peptides (CPPs), also known as protein transduction domains (PTDs) or membrane transduction peptides (MTPs) have been widely used for the intracellular uptake and delivery of various macromolecules, or "cargo", which are associated with the peptides either via covalent bond or through non-covalent interaction, such as TAT family peptides.
Although many peptide sequences are immunogenic, not all of them are identically effective in generating antibodies when act against the target protein. Effective antibody generation depends on several factors that need to be considered at the very first stage of peptides designing.
Peptide Microarrays perfectly complement peptide display screens (e.g. mRNA display, phage display or CIS display) in regard to peptide target binder development. Binding motifs can be further optimized by Single and Double Substitution Scans to increase the affinity of the peptide target binder.
Key factors include peptide sequence length, amino acid composition, and hydrophobic residues. These factors influence solubility, assembly, and purification feasibility.
De novo design allows for the creation of peptides with specific functions and structures. It involves an optimization-based sequence selection, fold specificity calculation, and binding affinity assessment to tailor peptides to your needs.
Our experts provide advice on overlap or offset of peptides, and using your full-length protein sequence, we generate a comprehensive peptide library for your targeted application. We also assist with experimental setup and analysis.
Stapled peptide design stabilizes peptides in an helical structure, enhancing their ability to bind efficiently to protein targets. This is particularly useful for disrupting protein-protein interactions in drug development.
CPPs are peptides that enable macromolecules to cross cell membranes, allowing for targeted intracellular delivery. These peptides are widely used in research for drug delivery, gene therapy, and intracellular imaging.
Effective peptide antigen design takes into account peptide length, hydrophilicity, and sequence composition to maximize immune response and ensure that the peptide induces the desired antibody generation against the target protein.
Peptide target binder development uses peptide microarrays to identify binding motifs. These motifs can be optimized using single and double substitution scans to enhance peptide affinity for specific targets.
Creative Peptides has extensive experience in peptide synthesis and design, with a team of experts that offer personalized support for complex peptide design challenges, ensuring high-quality and effective results.
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