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Custom Peptide Design
Interested in the use of biologically active proteins and peptides as potential therapeutic agents has grown dramatically in recent years. Although many meaningful studies can be performed, it is important to consider several factors such as the length of sequence, amino acid residues and the sequence of peptides, all of which will influence whether correct assembly and purification are feasible. If you would like Creative Peptides to help you in designing your peptide, our technical support department can assist you.
The following suggestions should be considered in the design of a peptide. They also determine the solubility of the final product.
- Reduce the length of sequence
- Reduce the number of hydrophobic residues
- Reduce difficult residues
- Change the sequence by choosing a different "framework"
Available Peptides Design Services
The full de novo peptide design framework is described in detail in the part of de novo peptide design. It consists of three stages: an optimization-based sequence selection, fold specificity calculation, and approximate binding affinity calculation.
Our technical and applications support team can offer advice on library design and experimental set up and analysis. Relating to design, we can advise on the best overlap or offset of peptides to be used in your target application, and then using your full-length protein sequence, we can generate the list of peptides for your library.
Stapling is a key technique for stabilizing peptides in an α-helical structure. The resultant stapled peptides are then able to compete efficiently for binding to protein targets involved in protein-protein interactions that are mediated by α-helices.
Cell-Penetrating Peptides (CPPs), also known as protein transduction domains (PTDs) or membrane transduction peptides (MTPs) have been widely used for the intracellular uptake and delivery of various macromolecules, or "cargo", which are associated with the peptides either via covalent bond or through non-covalent interaction, such as TAT family peptides.
Although many peptide sequences are immunogenic, not all of them are identically effective in generating antibodies when act against the target protein. Effective antibody generation depends on several factors that need to be considered at the very first stage of peptides designing.
Peptide Microarrays perfectly complement peptide display screens (e.g. mRNA display, phage display or CIS display) in regard to peptide target binder development. Binding motifs can be further optimized by Single and Double Substitution Scans to increase the affinity of the peptide target binder.
 Hosseinzadeh, P., Bhardwaj, G., Mulligan, V. K., Shortridge, M. D., Craven, T. W., Pardo-Avila, F. & Webb, I. K. (2017). Comprehensive computational design of ordered peptide macrocycles. Science, 358(6369), 1461-1466.
 Su, Z., Shen, H., Wang, H., Wang, J., Li, J., Nienhaus, G. U. & Wei, G. (2015). Motif‐Designed Peptide Nanofibers Decorated with Graphene Quantum Dots for Simultaneous Targeting and Imaging of Tumor Cells. Advanced Functional Materials, 25(34), 5472-5478.