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Peptide Mass Fingerprinting
Peptide Mass Fingerprinting (PMF) assay is a method for mass spectrometric analysis of a mixture of polypeptides obtained after proteolysis or degradation. The peptide obtained by mass spectrometry is compared with the theoretical peptide of the protein in the polypeptide protein database to determine whether the measured protein is known or unknown. Since different proteins have different amino acid sequences, the peptides obtained from different proteins have fingerprint characteristics. The absolute mass of each protein peptide is theoretically calculated to find the best match compared to the mass of the unknown protein peptide. A variety of proteomes have been studied using peptide fingerprinting methods.
The advantage of PMF is that if the protein is present in the protein database, only the mass of the protein peptide is known, and it takes no time to perform DEVONO sequencing. However, PMF-based protein identification relies on a single protein because mixed proteins can significantly complicate PMF analysis and may yield incorrect results. Therefore, for PMF identification with more than 2-3 protein samples, additional MS/MS-based protein identification is required to enhance the specificity of protein identification.
- Identification of protein gel sample
Creative Peptides accepts samples of various types of dyed protein tapes, including Camas Bright Blue, Silver, Fluorescent, Zn/Cu2+, and others. We have no minimum requirements for protein content in the sample. The analytical method used by us is the most sensitive and reliable method for identifying protein tapes isolated from gels (including two-dimensional gels) in the world.
- Identification of simple protein mixture
This service can be used to identify protein mixtures within 300 proteins. For example, protein complexes prepared by immunoprecipitation (antibodies should be cross-linked on a solid support), or protein complexes prepared by the Pull-down method. Compared to the identification of a single protein tape, this method can identify up to 300 proteins at a lower cost.
- Complete identification of protein mixtures
We can identify thousands of different proteins from a mixture sample and provide customers with complete protein data, to help customers obtain the complete protein data, especially low protein concentration, but very important.
- Many years of experience in mass spectrometry testing, quality assurance
- Optimized sample processing steps to maximize the integrity of protein information
- Proprietary system improvement techniques increase the amount of protein detection and increase the sensitivity of detection
- A dozen proprietary analysis software that ensure accurate results
1. Graves, P. R., & Haystead, T. A. (2002). Molecular biologist's guide to proteomics. Microbiology & Molecular Biology Reviews Mmbr, 66(1), 39.
2. Yang, D., Lü, X., Hong, Y., Xi, T., & Zhang, D. (2013). The molecular mechanism of mediation of adsorbed serum proteins to endothelial cells adhesion and growth on biomaterials. Biomaterials, 34(23), 5747-5758.
3. Alty, L. T., & Lariviere, F. J. (2016). Peptide mass fingerprinting of egg white proteins. Journal of Chemical Education, 93(4), págs. 772-777.