Peptide Nucleic Acid Synthesis

Designed for biological research and industrial applications, not intended for individual clinical or medical purposes.

PNAs, or peptide nucleic acids, is an analogue of oligonucleotide in which sugar-phosphate backbone has been replaced by a polyamide chain. Due to the unique backbone of PNA, it can be used in same applications as conventional DNA/RNA. Comparing with DNA, PNAs are neutral in charge, chemically more stable and resistant to DNases and proteases, as well as remaining high sequence specificity, binding activity and lower salt sensitivity. PNAs have been widely used in molecular diagnostics and therapeutics, such as fluorescent in situ hybridization (Fish), single nucleotide polymorphisms (SNPs) detection and antisense therapy.

Peptide Nucleic Acid Synthesis Service

Advantages of PNA Unique Backbone

PNA binds to DNA/RNA with higher affinity and greater specificity than conventional oligonucleotides
PNA is more stable than DNA or RNA as it is resistant to nucleases and proteases
PNA can be hydridized at lower salt concentrations than DNA or RNA which leads to higher stringency
PNA makes ideal reagents for antisense and antigene use, probes for molecular diagnostics, & microarrays

Applications of Peptide Nucleic Acid (PNA)

Microarrays and biosensors
PNA microarray combined with PCR could detect genetically modified organisms.
PNA FISH Probes
In fluorescent in situ hybridization (FISH), short probes can be difficult to detect (owing to insufficient hybridization and low levels of labelling), while longer probes can induce non-specific background fluorescence. In PNA-FISH, shorter PNA probes can be used (because of the higher specificity of PNA), resulting in less background noise. The fluorescent dye-conjugated PNA can bind to DNA or RNA quickly, even under low salt.
PCR Blockers
PNA probes have no direct interaction with DNA polymerase but PNAs can terminate the elongation of oligonucleotide primers by binding to the template or competing with the primers. Moreover, PNA-DNA chimeras can be recognized by the DNA polymerase and can thus be used as primers for PCR reactions. The high affinity binding of PNAs has also been used for detecting single base pair mutations by PCR. This strategy, named PNA directed PCR clamping, uses PNAs to inhibit the amplification of a specific target by direct competition of the PNA targeted against one of the PCR primer sites and the conventional PCR primer.
PNA miRNA Inhibitors
Compare to DNA/RNA, PNA has more strongly affinity when bind to complementary RNA. The character make PNA can be used as a miRNA inhibitors. Moreover, PNA miRNA inhibitors are conjugated to cell penetrating peptide (CPP), allowing them enter to cells without the need of transfection reagents.
Antisense and Antigene Drugs
PNA can bind to a complementary sequence of mRNA and change its function. PNA can break up DNA duplex and form PNA/DNA triplex or double duplexes without denaturing the DNA duplex. It can also inhibit the transcription and translation of target genes. Besides, PNA can improve the cellular delivery of PNA by binding of cell penetrating proteins (CPP). It has high sequence specifity for target in gene sequence.
Double Strand DNA Invasion and Capture
Because of its uncharged polyamide backbone, PNA can hybridize to negatively charged DNA or RNA without electrostatic repulsion.

We offer the many custom PNA synthesis services:

PNA synthesis by automated solid-phase methods, as with DNA and RNA oligonucleotide synthesis.
PNA Scales from 5mg to mutli-gram
PNAs from 3 to 100 monomers
PNA-Peptide conjugations
PNA-Protein conjugations
Specialized PNA modifications
PNA libraries
PNA Arrays
PNA Purification, PNA purities from crude to >98%

Competitive Advantages

Highly Customizable: Flexible synthesis scales, various modification types, and diversified delivery forms.
Excellent Stability: Each batch of synthetic products is strictly purified by HPLC in the RNase-free environment to avoid RNase contamination in the whole process.
High quality: Reliable lab report with timely updates. A RP-HPLC trace and Mass Spec analysis are included.
Technical Support: Professional team provides you with comprehensive pre-sale consultation, order design, and after-sales service to ensure the products deliver smoothly.
Cost-Effective: Competitive prices and short turnaround time.

Creative Peptides can provide a number of PNA related products, such as Gamma PNA or Fmoc/Boc PNA Monomers etc.

Fmoc-PNA-A(Bhoc)-OH

CAS: 186046-82-2

Chemical Formula: C40H35N7O7

Molecular Weight: 725.76

Fmoc-PNA-A(Boc)-OH

CAS: 511534-99-9

Chemical Formula: C31H33N7O7

Molecular Weight: 615.65

Boc-PNA-A(Z)-OH

CAS: 149376-69-2

Chemical Formula: C24H29N7O7

Molecular Weight: 527.54’

Fmoc-PNA-C(Bhoc)-OH

CAS: 186046-81-1

Chemical Formula: C39H35N5O8

Molecular Weight: 701.74

Fmoc-PNA-C(Boc)-OH

CAS: 172405-61-7

Chemical Formula: C30H33N5O8

Molecular Weight: 591.62

Boc-PNA-C(Z)-OH

CAS: 144564-94-3

Chemical Formula: C23H29N5O8

Molecular Weight: 503.51

Fmoc-PNA-G(Bhoc)-OH

CAS: 186046-83-3

Chemical Formula: C40H35N7O8

Molecular Weight: 741.76

Fmoc-PNA-G(Boc)-OH

CAS: 1052677-90-3

Chemical Formula: C31H33N7O8

Molecular Weight: 631.65

Boc-PNA-G(Z)-OH

CAS: 169287-77-8

Chemical Formula: C24H29N7O8

Molecular Weight: 543.54

Fmoc-PNA-T-OH

CAS: 169396-92-3

Chemical Formula: C26H26N4O7

Molecular Weight: 506.52

Boc-PNA-T-OH

CAS: 139166-80-6

Chemical Formula: C16H24N4O7

Molecular Weight: 384.39

Fmoc-PNA-U-OH

CAS: 959151-70-3

Chemical Formula: C25H24N4O7

Molecular Weight: 492.49

Boc-PNA-U-OH

CAS: 149500-74-3

Chemical Formula: C15H22N4O7

Molecular Weight: 370.36

Boc-PNA-thioU(PMB)-OH

CAS: 253438-99-2

Chemical Formula: C23H30N4O7S

Molecular Weight: 506.57

Fmoc-PNA-M(Bhoc)-OH

CAS: NA

Chemical Formula: C40H36N4O7

Molecular Weight: 684.75

Fmoc-PNA-M(Boc)-OH

CAS: 1417611-27-8

Chemical Formula: C31H34N4O7

Molecular Weight: 574.63

Boc-PNA-M(Z)-OH

CAS: NA

Chemical Formula: C24H30N4O7

Molecular Weight: 486.53

Fmoc-PNA-J(Bhoc)-OH

CAS: NA

Chemical Formula: C39H35N5O8

Molecular Weight: 701.74

Fmoc-PNA-J(Boc)-OH

CAS: 1095275-70-9

Chemical Formula: C30H33N5O8

Molecular Weight: 591.62

Boc-PNA-J(Z)-OH

CAS: 163081-03-6

Chemical Formula: C23H29N5O8

Molecular Weight: 503.51

Fmoc-PNA-D(tetraBhoc)-OH

CAS: NA

Chemical Formula: C82H66N8O13

Molecular Weight: 1371.47

Fmoc-PNA-D(tetraBoc)-OH

CAS: 2101661-88-3

Chemical Formula: C46H58N8O13

Molecular Weight: 931.01

Boc-PNA-D(tetraZ)-OH

CAS: NA

Chemical Formula: C48H48N8O13

Molecular Weight: 944.96

FAQs

1. What are peptide nucleic acids (PNAs) and how are they different from DNA or RNA?

PNAs are synthetic analogs of oligonucleotides where the sugar-phosphate backbone is replaced by a polyamide chain. Unlike DNA or RNA, PNAs are neutral in charge, more chemically stable, and resistant to nucleases and proteases. They exhibit higher sequence specificity and binding activity, making them ideal for various molecular applications.

2. What are the advantages of using PNAs in molecular applications?

PNAs have superior binding affinity and specificity to DNA and RNA compared to conventional oligonucleotides. They are more stable, less sensitive to salt concentrations, and resistant to nucleases, making them excellent for applications like antisense therapy, diagnostics, and microarrays.

3. What are some common applications of PNA?

PNAs are widely used in molecular diagnostics, including applications like PNA-FISH (fluorescent in situ hybridization) probes, PCR blockers, miRNA inhibitors, and antisense therapy. They are also useful in detecting genetically modified organisms and in the development of PNA arrays for high-throughput screening.

4. How does PNA-FISH work and what are its benefits?

PNA-FISH uses PNA probes conjugated with fluorescent dyes to detect DNA or RNA sequences. Due to PNA's high specificity, shorter probes can be used, resulting in less background noise and quicker hybridization, even under low-salt conditions, compared to traditional DNA probes.

5. How does PNA inhibit miRNA and what makes it effective?

PNAs bind more strongly to complementary RNA sequences than DNA or RNA, making them highly effective miRNA inhibitors. When conjugated with cell-penetrating peptides (CPPs), PNA miRNA inhibitors can enter cells without the need for transfection reagents, providing a powerful tool for gene regulation studies.

6. What is the role of PNAs in antisense and antigene therapies?

PNAs can bind to complementary mRNA sequences, preventing gene expression by forming PNA/DNA triplexes or double duplexes. This ability to block transcription and translation of target genes makes PNAs a valuable tool in gene silencing therapies and research.

7. How does PNA work in detecting single base pair mutations?

PNAs can inhibit PCR amplification by binding directly to one of the PCR primer sites, preventing the elongation of primers during PCR reactions. This method, known as PNA-directed PCR clamping, is particularly effective in detecting single base pair mutations by outcompeting the conventional PCR primers.

8. What services does Creative Peptides offer for PNA synthesis?

Creative Peptides offers a wide range of PNA synthesis services, including automated solid-phase synthesis, custom PNA scales from 5 mg to multi-grams, specialized modifications, PNA-peptide and PNA-protein conjugations, and PNA arrays. We also provide high-quality purification with HPLC and mass spectrometry analysis.