CEF Control Peptides

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CAT# Product Name M.W Molecular Formula Inquiry
C14002 CEF2, Influenza Virus PA (46-54) 1206.4 Inquiry
C14003 CEF3, Influenza Virus M1 (13-21) 911.1 Inquiry
C14004 CEF4, Influenza Virus NP (342-351) 1148.4 Inquiry
C14005 CEF5, Influenza Virus NP (91-99) 1019.2 Inquiry
C14006 CEF6, Influenza Virus NP 1051.3 Inquiry
C14007 CEF7, Influenza Virus NP (380-388) 1193.4 Inquiry
C14009 CEF9, Influenza VirusM1 128-135 857.1 Inquiry
C14010 CEF10, Epstein-Barr Virus LMP2 (426-434) 906.2 Inquiry
C14011 CEF11, Epstein-Barr Virus BMLF1 (280-288) 920.2 Inquiry
C14012 CEF12, Epstein-Barr Virus latent NA3B (399-408), AVF 1136.3 Inquiry
C14014 CEF15, Epstein-Barr Virus Lytic Cycle Protein BZLF-1(190-197) 1003.3 Inquiry
C14015 CEF16, Epstein-Barr Virus latent NA-3A (337-347) 1052.3 Inquiry
C14016 CEF17, Epstein-Barr Virus latent NA-3A (158-166) 1143.4 Inquiry
C14017 CEF18, Epstein-Barr Virus latent NA-3C (258-266) 1190.4 Inquiry
C14020 CEF21, Cytomegalovirus, CMV pp65 (417-426) 946.1 Inquiry
C14021 CEF22, Cytomegalovirus, CMV pp65 (378-389) 1339.4 Inquiry
C14022 CEF23, Cytomegalovirus, CMV pp65 (123-131) 1079.2 Inquiry
C14023 CEF24, Influenza Virus PB1 Peptide (591-599) 921 Inquiry
C14024 CEF25, Influenza Virus NP (44-52) 1071.2 Inquiry
C14025 CEF26, Influenza Virus NP (265-274) 980.2 Inquiry

The length of CEF (Cytomegalovirus, Epstein-Barr Virus, Influenza Virus) control peptide is 8 to 12 amino acids, and its sequence is derived from human cytomegalovirus, EB virus and influenza virus 1. These peptides are used to stimulate CD8+T cells to release IFN- γ. In the detection of many kinds of cytokines such as ELISPOT, they have important application value as positive control peptides. CEF peptides were first screened in 2002 based on their ability to recognize CD8+T cells. It comes from the antigenic epitope of the virus, so it has the antigenic property.

Structure of CEF peptide

CEF peptide is composed of 8 to 11 amino acids, and the sequence is as follows: GILGFVFTL (influenza A, HLA-A2), FMYSDFHFI (influenza A, HLA-A2), CLGGLLTMV (EBV, HLA-A2), GLCTLVAML (EBV, HLA-A2), NLVPMVATV (HCMV, HLA-A2).


CEF peptide can stimulate CD8+T cells to produce specific interferon-γ, which can be used as a positive control for the detection of γ-ELISA and F-Spot in human peripheral blood mononuclear cells (PBMCs). CEF control peptides are used as positive controls in ELISPOT tests to study specific immune responses to a variety of diseases, including infection, cancer, allergies and autoimmune diseases. In this case, the CEF peptide ensures that the cells in the study are active and visible. ELISPOT is also useful in developing vaccines, especially in HIV vaccines, where CEF peptides are also used as control.


  1. Xu, Y., Li, H., Gao, R. L., Adeyemo, O., Itkin, M., & Kaplan, D. E. (2011). Expansion of interferon-gamma-producing multifunctional CD4+ T-cells and dysfunctional CD8+ T-cells by glypican-3 peptide library in hepatocellular carcinoma patients. Clinical Immunology, 139(3), 302-313.
  2. Pohlmeyer, C. W., Laskey, S. B., Beck, S. E., Xu, D. C., Capoferri, A. A., Garliss, C. C., ... & Leffell, M. S. (2018). Cross-reactive microbial peptides can modulate HIV-specific CD8+ T cell responses. PloS one, 13(2), e0192098.
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