AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.
Peptide Library Construction and Screening
Powerful screening tools in biological and chemical research
M.W/Mr. | 729.7 |
Sequence | One Letter Code: Ac-IETD-AFC Three Letter Code: Ac-Ile-Glu-Thr-Asp-AFC |
References | 1. Gurtu V, et al. (1997). Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal Biochem 251, 98-102; 2. Martins LM, et al. (1997). Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J Biol Chem 272, 7421-30; 3. Deveraux QL, et al. (1997). X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388, 300-4; 4. Xiang J, et al. (1996). BAX-induced cell death may not require interleukin 1 ?-converting enzyme-like proteases. Proc Natl Acad Sci USA 93, 14559-63. |
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at info@creative-peptides.com. We will endeavor to provide highly satisfying products and services.