AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.
CAT# | A55002 |
M.W/Mr. | 729.7 |
Sequence | One Letter Code: Ac-IETD-AFC Three Letter Code: Ac-Ile-Glu-Thr-Asp-AFC |
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