Secretin stimulates the secretion of bicarbonate by the pancreas and inhibits the gastrin and acid production in the stomach. It also potentiates the release of digestive enzymes from the pancreas triggered by cholecystokinin.
CAT No: 10-101-99
CAS No:17034-35-4 (net)
Synonyms/Alias:RG 1068; RG1068; RG-1069; Secretin
Secretin (porcine) Hydrochloride is a peptide hormone derived from porcine sources, widely recognized for its role in regulating pancreatic secretions and facilitating digestive processes. As a member of the secretin family of peptides, it is composed of 27 amino acids and functions primarily as a key modulator of bicarbonate secretion in the pancreas, as well as influencing the activity of other gastrointestinal organs. In biochemical research, this compound is valued for its ability to mimic endogenous secretin activity, enabling detailed studies of peptide hormone signaling, receptor interactions, and gastrointestinal physiology. Its well-characterized structure and conserved biological activity across species make it a critical tool for dissecting the complex regulatory pathways that govern exocrine and endocrine functions in mammalian systems.
Receptor binding studies: Secretin (porcine) Hydrochloride is extensively employed in receptor binding assays aimed at characterizing secretin receptor subtypes and their pharmacological profiles. By providing a reliable ligand for the secretin receptor, it allows researchers to investigate receptor-ligand interactions, assess binding affinities, and map receptor distribution in various tissues. These studies are fundamental for elucidating the molecular mechanisms underlying hormone-receptor specificity and for advancing the understanding of G protein-coupled receptor signaling pathways.
Signal transduction research: The peptide is instrumental in experiments designed to dissect intracellular signaling cascades initiated by secretin receptor activation. Upon binding to its receptor, secretin triggers cyclic AMP (cAMP) production and activates downstream effectors involved in ion transport and enzyme secretion. Utilizing this compound in cell-based assays or organ preparations enables the detailed analysis of second messenger systems, kinase activation, and cross-talk with other hormonal pathways, providing insight into the integrated control of gastrointestinal and metabolic processes.
Gastrointestinal physiology models: In studies of digestive system function, porcine secretin hydrochloride serves as a physiological stimulant to probe the regulation of pancreatic and biliary secretions. It is routinely used to induce and quantify bicarbonate-rich fluid secretion from pancreatic acinar cells, supporting investigations into the mechanisms of exocrine pancreas regulation, as well as the pathophysiology of pancreatic insufficiency and related disorders in animal models. This application is central to advancing knowledge of digestive enzyme regulation and the interplay between neural and hormonal control in the gut.
Peptide structure-activity relationship (SAR) analysis: The defined sequence and biological activity of this peptide make it an ideal reference compound for structure-activity relationship studies within the secretin/glucagon peptide family. Researchers utilize it to compare activity profiles of natural and synthetic secretin analogs, assess the impact of amino acid substitutions, and delineate critical structural motifs responsible for receptor activation. These investigations inform rational peptide design and facilitate the development of novel ligands with tailored properties for research or diagnostic applications.
Analytical method development: Secretin (porcine) Hydrochloride is also employed as a standard or calibration reference in the development and validation of analytical techniques for peptide quantification. Its use supports the optimization of high-performance liquid chromatography (HPLC), mass spectrometry, and immunoassay protocols aimed at detecting and measuring peptide hormones in biological samples. Accurate quantification of secretin and related peptides is essential for endocrinological research, biomarker discovery, and studies of peptide stability, metabolism, and pharmacokinetics.
Gastric acid secretion is under nervous and hormonal control. Gastrin, the major circulating stimulus of acid secretion, probably does not stimulate the parietal cells directly but acts to mobilize histamine from the ECL cells in the oxyntic mucosa. Histamine stimulates the parietal cells to secrete HCl. The gastrin-ECL cell pathway has been investigated extensively in situ (gastric submucosal microdialysis), in vitro (isolated ECL cells) and in vivo (intact animals). Gastrin acts on CCK2 receptors to control the synthesis of ECL-cell histamine, accelerating the expression of the histamine-forming enzyme histidine decarboxylase (HDC) at both the transcription and the translation/posttranslation levels. Depletion of histamine by alpha-fluoromethylhistidine (an irreversible inhibitor of HDC) prevents gastrin-induced but not histamine-induced gastric acid secretion. Acute CCK2 receptor blockade inhibits gastrin-evoked but not histamine-induced acid secretion. Studies both in vivo/in situ and in vitro have suggested that while acetylcholine seems capable of activating parietal cells, it does not affect histamine secretion from ECL cells. Unlike acetylcholine, the neuropeptides pituitary adenylate cyclase-activating peptide and vasoactive intestinal peptide mobilize ECL-cell histamine. Whether vagally stimulated acid secretion reflects an effect of the enteric nervous system on the ECL cells (neuropeptides) and/or a direct one on the parietal cells needs to be further investigated.
Lindström, E., Chen, D., Norlén, P., Andersson, K., & Håkanson, R. (2001). Control of gastric acid secretion: the gastrin-ECL cell-parietal cell axis. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 128(3), 503-511.
Our understanding of secretin and pancreatic secretions has developed. However, the salient findings of Bayliss and Starling concerning secretin have stood the test of time. Thus secretin is recognized as the key pathway mediating a now classical negative feedback reflex. Acidic chyme, emptying from the stomach, stimulates upper small intestinal mucosal S cells to release secretin. Secretin stimulates the flow of a bicarbonate-rich pancreatic secretion which empties into the duodenum, thus neutralizing the acid chime and removing the stimulus for secretin release. Of wider significance, the concept of hormonal regulation and the definition of a hormone basically remain to this day as originally outlined in Starling's Croonian Lectures.
Hirst, B. H. (2004). Secretin and the exposition of hormonal control. The Journal of physiology, 560(2), 339-339.
1. Store-operated Ca2+ entry sustains the fertilization Ca2+ signal in pig eggs
3. Cell-based adhesion assays for isolation of snake venom’s integrin antagonists
5. An Open-label, Single-center, Safety and Efficacy Study of Eyelash Polygrowth Factor Serum
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More