DABCYL-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS trifluoroacetate salt functions as a FRET-based protease substrate. DABCYL and EDANS form a quencher-fluorophore pair whose separation reports on peptide cleavage. The sequence is optimized for sensitive kinetic measurements in solution. Researchers use it in real-time protease assays, inhibitor screening, and mechanistic enzymology.
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