Fmoc-Gly-OH-2-¹³C,¹⁵N is an Fmoc-protected glycine derivative bearing isotopic labels at the glycine backbone carbon and nitrogen (¹³C,¹⁵N). The molecule contains an Fmoc carbamate protecting group on the amino functionality while retaining a free carboxylic acid, enabling controlled chemoselective handling during stepwise peptide assembly; the isotopic substitution provides a distinct NMR or mass spectrometric signature for analytical tracking. Employed as a labeled amino acid building block in solid-phase peptide synthesis and related labeling workflows, it supports preparation of isotopically enriched peptides and peptide fragments for structural analysis, quantitative mass spectrometry, and isotopic tracing studies.
CAT No: HB00067
Fmoc-Gly-OH-2-¹³C,¹⁵N is a glycine building block bearing the Fmoc (9H-fluoren-9-ylmethoxycarbonyl) protecting group on the amino functionality and a stable isotope label at the glycine backbone. The ¹³C,¹⁵N enrichment enables mass-shifted detection in peptide and protein workflows, while the Fmoc-protected format supports standard protected-amino-acid handling during peptide assembly. This reagent is commonly used when isotopically defined glycine is needed for quantitative LC-MS peptide standards, labeled peptide synthesis, and isotope-resolved chemical biology experiments.
1. Isotopic Peptide Standards
Fmoc-Gly-OH-2-¹³C,¹⁵N is used to incorporate a defined ¹³C,¹⁵N glycine unit into peptides that serve as quantitative LC-MS standards or calibration references. Researchers in proteomics and targeted mass spectrometry workflows rely on isotopically labeled peptide surrogates to improve signal assignment and quantitation robustness, especially when monitoring glycine-containing tryptic or non-tryptic peptides. The Fmoc-protected amino acid format allows consistent incorporation into custom peptide sequences, yielding standards with predictable mass shifts and reduced ambiguity during spectral matching.
2. Stable-Isotope Proteomics Workflows
Fmoc-Gly-OH-2-¹³C,¹⁵N supports stable isotope-resolved protein and proteome studies where labeled peptide fragments are used for quantification and method development. Proteomics groups often synthesize isotopically labeled internal standards to normalize instrument response across runs and to validate chromatographic behavior for specific peptide targets. By using an Fmoc-compatible glycine building block with backbone ¹³C,¹⁵N labeling, developers can generate reference peptides that report on the labeled residue with high confidence in MS/MS-based assays, including workflows that require carefully defined isotopic composition rather than unlabeled analogs.
3. Labeled Peptide Synthesis
Fmoc-Gly-OH-2-¹³C,¹⁵N is applied in custom peptide synthesis when a glycine residue must be isotopically labeled while maintaining the operational advantages of Fmoc chemistry. Peptide chemistry teams use this reagent to prepare sequence-defined labeled peptides for analytical studies, binding assays, and chemical biology probes where residue-level isotopic identity is important for downstream readout. The Fmoc protection enables routine solid-phase peptide synthesis handling and purification-compatible workflows, allowing the labeled glycine to be positioned precisely within a peptide sequence for residue-specific tracking by mass spectrometry.
4. Quantitative LC-MS Method Development
Fmoc-Gly-OH-2-¹³C,¹⁵N is frequently selected for LC-MS method development and verification experiments that require isotopically distinct analytes. Analytical laboratories use labeled peptides containing ¹³C,¹⁵N glycine to evaluate chromatographic separation, ionization behavior, and fragmentation patterns under defined instrument conditions. Because the isotope label is built into the peptide at the residue level during synthesis, the resulting standards provide consistent mass signatures for tuning acquisition parameters and for validating data processing pipelines that rely on isotope-aware peak identification.
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