PTD2 is a protein transduction domain peptide enriched in basic residues that support efficient cellular uptake. The sequence interacts with anionic membrane components, enabling translocation into the cytosol. Researchers investigate its cargo-delivery efficiency and structural dynamics. Applications include intracellular delivery design, CPP optimization, and mechanistic membrane studies.
CAT No: R2792
Synonyms/Alias:PTD2; CHEMBL5402803; HY-P5922; BDBM50627878; CS-0896328;
PTD2, also known as Protein Transduction Domain 2, is a synthetic peptide sequence recognized for its cell-penetrating capabilities. As a member of the protein transduction domain family, PTD2 is engineered to facilitate the intracellular delivery of various molecular cargoes, including peptides, proteins, nucleic acids, and other bioactive compounds. Its amphipathic structure and positive charge distribution enable it to traverse cellular membranes efficiently, making it a valuable tool in biochemical research focused on manipulating and understanding intracellular processes. The functional versatility of PTD2 has garnered significant interest in molecular biology, biochemistry, and cell biology, where controlled delivery of biomolecules is essential for elucidating cellular mechanisms and developing innovative research methodologies.
Intracellular delivery studies: PTD2 is widely employed in research aimed at enhancing the cellular uptake of diverse biomolecules. By conjugating PTD2 to target proteins, peptides, or nucleic acids, researchers can overcome the intrinsic barrier posed by the plasma membrane, enabling efficient transport of these molecules into the cytosol. This property is particularly valuable in studies investigating protein function, signal transduction, and gene regulation, as it allows for precise manipulation of intracellular environments without the need for invasive techniques or chemical transfection reagents.
Peptide-based assay development: The unique cell-penetrating properties of PTD2 make it a preferred choice for developing peptide-based bioassays. In such applications, PTD2-tagged peptides can be used to deliver functional motifs or reporter sequences directly into living cells, facilitating the real-time monitoring of enzymatic activity, protein-protein interactions, or cellular responses to external stimuli. This approach significantly expands the toolkit available for high-content screening and mechanistic studies in both basic and applied research settings.
Molecular imaging and labeling: PTD2 can be conjugated to fluorescent dyes, imaging probes, or bioluminescent markers to enhance their intracellular localization in live cell imaging experiments. By enabling efficient delivery of these labeled probes, researchers can achieve high-resolution visualization of subcellular structures, track dynamic processes, or quantify molecular events within intact cells. This application supports advanced imaging workflows in cell biology, neuroscience, and pharmacology, where accurate spatial and temporal information is critical.
Functional genomics and gene editing: The use of PTD2 as a delivery vector extends to the field of functional genomics, where it can facilitate the transport of antisense oligonucleotides, siRNAs, or CRISPR-associated proteins into target cells. This capability allows for efficient modulation of gene expression, targeted gene knockdown, or genome editing without relying on viral vectors or electroporation. As a result, PTD2 contributes to the development of streamlined protocols for dissecting gene function and validating genetic targets in a variety of cell types.
Protein engineering and synthetic biology: Researchers in protein engineering and synthetic biology leverage PTD2 to design modular protein constructs with enhanced delivery and functionalization properties. By incorporating PTD2 sequences into recombinant proteins or fusion constructs, it is possible to endow these molecules with intrinsic membrane-translocating abilities. This strategy enables the creation of novel biosensors, intracellular enzymes, or regulatory factors that can be directly introduced into living cells, supporting the advancement of synthetic biology platforms and the exploration of new cellular engineering paradigms.
1. An Open-label, Single-center, Safety and Efficacy Study of Eyelash Polygrowth Factor Serum
3. Emu oil in combination with other active ingredients for treating skin imperfections
4. Urinary Metabolites Associated with Blood Pressure on a Low-or High-Sodium Die
5. Store-operated Ca2+ entry sustains the fertilization Ca2+ signal in pig eggs
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More