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B-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, is isolated from cyanobacteria and eukaryotic algae. Its primary absorption peak is at 545 nm with a secondary peak at 563 nm. B-PE consists of a, b and g subunits and is present as (ab)6g. B-PE and the closely related R-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. B-PE labeled streptavidin, primary and secondary antibodies have been widely used in applications such as flow cytometry and multi-color immunofluorescent staining. The CPK B - PE Labeling Kit contains SH-reactive B-PE. SMCC modified B-PE reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol.
Peptide Library Construction and Screening
Powerful screening tools in biological and chemical research
Labeling Target | Antibodies (General) |
Size | 1 kit:1 labeling reaction (1x1 mg IgG) |
Conjugate | B-PE |
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