Bacterial Sortase Substrate III, Abz/DNP

Bacterial Sortase Substrate III, Abz/DNP is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.

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CAT#	R1231
M.F/Formula	C₄₁H₅₇N₁₁O₁₄
M.W/Mr.	928.00
Sequence	One Letter Code: Abz-LPETG-K(Dnp)-NH2 three Letter Code: Abz-Leu-Pro-Glu-Thr-Gly-Lys(Dnp)-NH2

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Storage	-20°C
References	Bentley, M. et al. J. Biol. Chem. 282, 6571 (2007).