Bim BH3, Peptide IV is a 26-residue peptide from BH3-only protein Bim, which belongs to the pro-apoptotic group of the Bcl-2 family of proteins.
CAT No: R1237
CAS No:2088247-34-9
Synonyms/Alias:Bim BH3, Peptide IV;721885-31-0;PD166913;G13743;
Bim BH3, Peptide IV is a synthetic peptide derived from the BH3 domain of the Bcl-2-interacting mediator of cell death (Bim) protein, a critical pro-apoptotic member of the Bcl-2 family. This peptide mimics the functional region of Bim responsible for antagonizing anti-apoptotic proteins and initiating programmed cell death. By recapitulating the key α-helical BH3 motif, Bim BH3, Peptide IV serves as a valuable molecular tool for dissecting protein-protein interactions central to apoptosis regulation. Its defined sequence and structural fidelity make it instrumental in advancing understanding of mitochondrial outer membrane permeabilization and the broader Bcl-2 family signaling network.
Apoptosis pathway research: Bim BH3, Peptide IV is widely utilized in studies investigating the molecular mechanisms of apoptosis, particularly the intrinsic pathway mediated by Bcl-2 family proteins. By introducing this peptide into cell-free systems or cellular models, researchers can probe the direct activation of pro-apoptotic effectors such as Bax and Bak, as well as the inhibition of anti-apoptotic proteins like Bcl-2 and Bcl-xL. This enables precise mapping of the signaling events leading to mitochondrial outer membrane permeabilization and downstream caspase activation, thereby clarifying the fundamental steps in programmed cell death.
Protein-protein interaction assays: The peptide is frequently employed as a probe in binding studies to characterize the affinity and specificity of BH3 domain interactions with various Bcl-2 family members. Techniques such as fluorescence polarization, surface plasmon resonance, and isothermal titration calorimetry benefit from the defined nature of Bim BH3, Peptide IV, allowing quantitative assessment of binding kinetics and competitive inhibition. These applications are crucial for elucidating the structural determinants of apoptotic regulation and for validating the functional relevance of mutant or engineered Bcl-2 proteins.
Peptide-based inhibitor development: Bim BH3, Peptide IV serves as a reference ligand in the rational design and screening of small molecule or peptidomimetic inhibitors targeting anti-apoptotic Bcl-2 proteins. By providing a structural and functional template, it aids in the identification of compounds that can disrupt pathogenic protein-protein interactions implicated in cell survival and resistance to apoptosis. This approach underpins the development of novel chemical probes and research tools for modulating cell fate in various experimental contexts.
Structural biology studies: The sequence and conformation of Bim BH3, Peptide IV make it an ideal candidate for co-crystallization or NMR studies with Bcl-2 family proteins. Structural analyses using this peptide help reveal the atomic details of BH3 domain recognition, allosteric modulation, and induced-fit binding mechanisms. Such insights contribute to a deeper understanding of the molecular architecture underlying apoptotic regulation and facilitate the rational engineering of improved peptide analogs or inhibitors.
Cell signaling modulation: In experimental systems, exogenous application of Bim BH3, Peptide IV is used to selectively trigger or sensitize cells to apoptosis, thereby enabling controlled perturbation of survival pathways. This functional property is leveraged to study cross-talk between apoptotic and non-apoptotic signaling cascades, to investigate the consequences of Bcl-2 family protein dysregulation, and to evaluate the impact of genetic or pharmacological interventions. Such applications provide valuable context for interpreting cellular responses to stress and for modeling disease-relevant phenotypes in vitro.
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