Caspase-3 Inhibitor III offers a peptide-based scaffold engineered for studying proteolytic pathways and substrate recognition. Its structural features enable detailed mapping of cleavage determinants. Researchers evaluate its conformational preferences to understand residue-driven specificity. Use includes mechanism studies, peptide optimization, and biochemical regulation modeling.
CAT No: R2442
CAS No:285570-60-7
Synonyms/Alias:Caspase-3 Inhibitor III;285570-60-7;Ac-DEVD-CMK;(4S)-4-[[(2S)-2-acetamido-3-carboxypropanoyl]amino]-5-[[(2S)-1-[[(2S)-1-carboxy-4-chloro-3-oxobutan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-oxopentanoic acid;CHEMBL1782265;Ac-Asp-Glu-Val-Asp-chloromethylketone trifluoroacetate salt;N-acetyl-Asp-Glu-Val-Asp-chloromethylketone;Ac-DEVD-CMK (trifluoroacetate salt);MFCD25372552;EX-A8700;HY-P0034;BDBM50346373;Ac-DEVD-CMK/Caspase-3 Inhibitor III;BP-23540;DA-70475;FA111126;acetyl-Asp-Glu-Val-Asp-chloromethyl ketone;CS-0014223;(2S,5S,8S,11S)-8-(2-carboxyethyl)-11-(carboxymethyl)-2-(2-chloroacetyl)-5-isopropyl-4,7,10,13-tetraoxo-3,6,9,12-tetraazatetradecane-1-carboxylic acid;(4S,7S,10S,13S)-7-(2-Carboxyethyl)-4-(carboxymethyl)-13-(2-chloroacetyl)-10-isopropyl-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid;
Caspase-3 Inhibitor III is a synthetic small molecule compound known for its potent and selective inhibition of caspase-3, a key cysteine protease involved in the execution phase of apoptosis. As a critical regulator of programmed cell death, caspase-3 plays a central role in cellular homeostasis, tissue remodeling, and the elimination of damaged or unwanted cells. The precise modulation of caspase-3 activity is essential for dissecting apoptosis pathways, understanding cell fate decisions, and exploring mechanisms underlying neurodegeneration, cancer, and other pathophysiological conditions. Caspase-3 Inhibitor III provides researchers with a valuable tool for investigating the molecular details of apoptotic signaling and for developing novel strategies in cell biology and molecular pharmacology.
Apoptosis pathway analysis: In studies aiming to elucidate the molecular mechanisms of programmed cell death, Caspase-3 Inhibitor III is routinely employed to selectively block caspase-3 activity. By inhibiting this executioner protease, researchers can distinguish between caspase-dependent and caspase-independent pathways, facilitating the dissection of upstream and downstream events in apoptosis. This approach enables the mapping of intricate signaling cascades and the identification of critical regulatory nodes within the cell death machinery.
Cell viability and survival assays: The compound is frequently utilized in cell culture experiments to assess the contribution of caspase-3-mediated apoptosis to overall cell viability. By preventing the activation of caspase-3, investigators can evaluate the protective effects of various stimuli or genetic modifications, as well as examine compensatory survival pathways. This application is particularly valuable for screening cytoprotective agents, investigating mechanisms of drug resistance, and optimizing culture conditions for sensitive cell types.
Neurodegeneration research: Caspase-3 Inhibitor III serves as an important experimental tool in the study of neurodegenerative disorders, where aberrant apoptosis contributes to neuronal loss. By selectively inhibiting caspase-3, researchers can model the effects of impaired apoptotic signaling in neuronal cultures or tissue models, providing insights into disease progression and the molecular basis of neuroprotection. This strategy supports the identification of novel therapeutic targets and the development of neuroprotective interventions at the preclinical research stage.
Mechanistic studies of protease specificity: The inhibitor is also employed to interrogate the substrate specificity and regulation of caspase family proteases. By selectively targeting caspase-3, scientists can differentiate its activity from that of other caspases, such as caspase-7 or caspase-9, within complex biological samples. This specificity allows for the characterization of distinct proteolytic events, the validation of novel substrates, and the clarification of overlapping or redundant functions among caspases.
Drug discovery and screening: In high-throughput screening platforms, Caspase-3 Inhibitor III is used as a reference compound or a tool to validate hits that modulate apoptotic processes. Its well-characterized inhibitory profile enables the benchmarking of novel chemical entities and the assessment of their selectivity and efficacy in modulating apoptosis. This application supports the identification and optimization of candidate molecules for further development in apoptosis-related research and drug discovery programs.
4. An Open-label, Single-center, Safety and Efficacy Study of Eyelash Polygrowth Factor Serum
5. SERS spectrum of the peptide thymosin‐β4 obtained with Ag nanorod substrate
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