3,5-Diiodo-L-thyronine is an iodinated, non-proteinogenic amino acid derivative related to thyronine, bearing a phenolic aromatic ring system and an amino acid side chain that includes both an amino group and a carboxyl group. The molecule contains two iodine substituents on the aromatic rings and is specified as the L-stereochemical form, with the side-chain functional groups enabling it to exist as a free amino acid or form amino acid salts depending on the counterion used. In biochemical and chemical biology research, it is used as a structurally defined iodinated building block for studying iodine-dependent properties, preparing thyronine analogs, and supporting analytical method development or labeling workflows where the presence and position of iodine atoms are chemically informative.
CAT No: CP18301
3,5-Diiodo-L-thyronine is an iodinated thyroid hormone amino-acid derivative featuring a L-thyronine backbone substituted with two iodine atoms at the 3 and 5 positions. This halogenated phenolic/aryl system makes it a widely used chemical reference for thyroid-related analytical workflows and for building blocks in iodine-substituted hormone chemistry. Because the compound is structurally defined and highly specific, it is frequently selected for method development, calibration, and downstream synthesis of iodinated thyronine analogs used in research-grade endocrine and chemical biology studies.
1. Thyroid Hormone Analytics
3,5-Diiodo-L-thyronine is used as a defined standard and reference material in analytical method development and quantification workflows targeting iodinated thyronines. Laboratories performing LC-MS/MS, HPLC, or immunoassay-related cross-reactivity mapping commonly rely on structurally specific iodinated standards to establish retention behavior, calibration curves, and identification criteria for thyroid hormone panels. Its 3,5-diiodo substitution pattern supports unambiguous discrimination among related iodinated species, which is particularly valuable when developing robust analytical panels for complex matrices such as serum, tissue extracts, or synthetic mixtures used in method validation.
2. Iodinated Thyronine Intermediate Synthesis
3,5-Diiodo-L-thyronine serves as a practical intermediate building block for preparing iodinated thyronine derivatives and analogs used in structure-activity and structure-property studies. Synthetic and medicinal chemistry groups use it to access defined iodine patterns on the thyronine scaffold, enabling controlled variation of aryl substitution patterns while keeping the core amino-acid-derived framework consistent. This makes the compound useful for generating research reagents and reference materials that support comparative studies across iodinated hormone analogs, including analog libraries intended for analytical cross-checks or chemical biology probe development.
3. Endocrine Research Reagent
3,5-Diiodo-L-thyronine is employed in endocrine-focused chemical biology workflows where defined iodinated thyronine chemistry is required rather than bulk thyroid extracts. Researchers use it to support experimental design involving thyroid-hormone analogs, including studies that require precise dosing of a single, structurally characterized iodinated species in in vitro systems or model mixtures. Its stable, well-defined substitution pattern helps ensure reproducibility when comparing responses across related iodinated compounds, and it is also commonly used as a benchmark reagent in experiments that evaluate chemical stability, interconversion behavior, or analytical detectability of thyronine-like species in controlled conditions.
4. Mass Spectrometry Reference Material
3,5-Diiodo-L-thyronine is frequently selected as a reference compound for mass spectrometric identification workflows involving iodinated thyroid metabolites and related standards. Analytical chemistry teams use it to confirm fragmentation behavior and to support spectral library building or targeted method tuning for iodinated aromatic species. Because the molecule carries two iodine atoms on the thyronine scaffold, it provides a distinctive mass signature that improves confidence in assignment during method development and routine quality checks for thyroid-hormone-related analytical panels.
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