Histone cluster 2, H2bf isoform b (108-126)

Histone cluster 2, H2bf isoform b (108-126) is a synthetic peptide fragment derived from the C-terminal region of the H2B family of histone proteins, specifically corresponding to residues 108 through 126 of the H2bf isoform. As a component of the nucleosome core, H2B plays a pivotal role in chromatin organization, gene regulation, and epigenetic signaling. The defined sequence of this peptide enables precise biochemical interrogation of histone modifications, protein-protein interactions, and the molecular mechanisms underlying chromatin dynamics. Its relevance extends across epigenetics, structural biology, and the development of analytical assays targeting histone function and modification.

Epigenetic research: Researchers frequently utilize the H2bf (108-126) peptide in studies investigating post-translational modifications (PTMs) of histone tails, such as acetylation, methylation, or ubiquitination. By providing a defined substrate that mimics the native histone context, this peptide facilitates the identification and characterization of enzymes responsible for these modifications, including histone methyltransferases, acetyltransferases, and deubiquitinases. Such studies are critical for unraveling the regulatory networks that govern chromatin accessibility and gene expression.

Protein interaction studies: The synthetic peptide serves as a valuable tool for mapping and quantifying interactions between histone tails and chromatin-associated proteins, including transcription factors, chromatin remodelers, and histone chaperones. Its use in binding assays, such as pull-down experiments or surface plasmon resonance, allows researchers to dissect the specificity and affinity of protein-histone interactions, contributing to a deeper understanding of chromatin assembly and regulation.

Antibody validation: The defined sequence of the H2bf (108-126) peptide makes it an effective reagent for validating the specificity and sensitivity of antibodies targeting histone H2B or its post-translationally modified forms. By serving as a positive or negative control in immunoassays such as western blotting, ELISA, or immunoprecipitation, it enables rigorous assessment of reagent performance and helps ensure the reliability of downstream analytical results.

Mass spectrometry standards: In proteomic workflows, synthetic histone peptides are routinely employed as calibration standards or internal controls for mass spectrometry-based quantification of histone modifications. The H2bf (108-126) fragment supports the accurate detection and quantification of both unmodified and modified histone peptides, improving the sensitivity, reproducibility, and interpretability of global or targeted histone proteomics studies.

Peptide-based assay development: The unique sequence and structural features of this histone fragment allow it to be incorporated into custom assay platforms designed to monitor the activity of chromatin-modifying enzymes or to screen for small molecule modulators of histone function. By enabling the development of high-throughput, biochemically defined assays, the peptide supports drug discovery and functional screening efforts focused on epigenetic targets relevant to disease biology and fundamental cellular processes.

1. Glucagonlike peptide 2 analogue teduglutide: stimulation of proliferation but reduction of differentiation in human Caco-2 intestinal epithelial cells

2. Myotropic activity and immunolocalization of selected neuropeptides of the burying beetle Nicrophorus vespilloides (Coleoptera: Silphidae)

3. Store-operated Ca2+ entry sustains the fertilization Ca2+ signal in pig eggs

4. Tachykinin-related peptides modulate immune-gene expression in the mealworm beetle Tenebrio molitor L

5. SERS spectrum of the peptide thymosin‐β4 obtained with Ag nanorod substrate