Myr-tat-CBD3 integrates an N-terminal myristoyl group, a TAT cell-penetration motif, and a CBD3 binding sequence for improved intracellular targeting. The myristoyl chain enhances membrane association, while basic residues facilitate translocation. Researchers study its interaction with cytoskeletal proteins and signaling complexes. Applications include delivery-peptide engineering, intracellular targeting, and protein-protein interaction modulation.
CAT No: R2771
Myr-tat-CBD3 is a synthetic peptide that combines a myristoylation modification, the HIV-1 tat protein transduction domain, and the collapsin response mediator protein 2 (CRMP2)-binding domain 3. This innovative molecular design enables effective cellular penetration and targeted modulation of protein-protein interactions, particularly those involving CRMP2. The peptide's amphipathic properties, imparted by myristoylation, facilitate membrane association, while the tat sequence enhances cellular uptake, making Myr-tat-CBD3 a versatile research tool for dissecting intracellular signaling pathways. Its unique structure allows researchers to manipulate CRMP2-related processes in diverse experimental systems, thereby advancing the understanding of neuronal development, cytoskeletal dynamics, and intracellular trafficking.
Neuroscience research: Myr-tat-CBD3 is widely used to investigate the role of CRMP2 in neuronal signaling and axonal guidance. By delivering the CBD3 domain directly into neurons, researchers can selectively disrupt CRMP2 interactions with its binding partners, such as voltage-gated calcium channels. This targeted interference provides valuable insights into the molecular mechanisms underlying neuronal plasticity, synaptic transmission, and the formation of neural circuits. Utilizing this peptide in in vitro or ex vivo neuron cultures allows for precise temporal and spatial control, enabling studies of axonal outgrowth, dendritic branching, and synaptic remodeling in response to experimental manipulations.
Ion channel modulation: The peptide is instrumental in probing the regulation of voltage-gated ion channels, particularly N-type calcium channels, by CRMP2. By competitively inhibiting the association between CRMP2 and these channels, Myr-tat-CBD3 facilitates the study of ion channel trafficking, surface expression, and functional modulation. This application is especially relevant in elucidating the cellular mechanisms that govern neurotransmitter release and neuronal excitability. Researchers leverage this tool to dissect the contributions of CRMP2-channel interactions to physiological and pathological signaling in neurons and other excitable cells.
Protein interaction mapping: Myr-tat-CBD3 serves as a valuable probe for mapping protein-protein interactions involving CRMP2. By selectively blocking or altering specific binding events, the peptide enables researchers to delineate the interaction networks that CRMP2 participates in within the cell. This approach aids in identifying novel CRMP2-associated proteins and characterizing their roles in cytoskeletal organization, intracellular transport, and signaling cascades. The ability to modulate these interactions in living cells enhances the resolution of interactome studies and supports the development of mechanistic models for CRMP2 function.
Cellular trafficking studies: The peptide's cell-permeable design makes it a powerful tool for investigating intracellular trafficking pathways regulated by CRMP2. Researchers utilize Myr-tat-CBD3 to disrupt CRMP2-dependent transport of vesicles, organelles, and membrane proteins, thereby clarifying the molecular machinery involved in endocytosis, exocytosis, and axonal transport. These studies contribute to a deeper understanding of how cytoskeletal dynamics and motor protein interactions are coordinated within the cell, with implications for neuronal polarity, synaptic function, and cellular homeostasis.
Drug discovery research: Myr-tat-CBD3 is increasingly employed in early-stage drug discovery as a molecular probe for validating CRMP2 as a therapeutic target. By modulating CRMP2 activity in cellular and animal models, researchers can assess the downstream effects on signaling pathways relevant to neurological disorders and pain mechanisms. This application supports the identification of small molecules or biologics that mimic or enhance the peptide's effects, accelerating the translation of basic research findings into potential therapeutic strategies. The versatility and specificity of Myr-tat-CBD3 thus make it an indispensable reagent for advancing fundamental and translational research in the life sciences.
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