Myr-tat-CBD3 integrates an N-terminal myristoyl group, a TAT cell-penetration motif, and a CBD3 binding sequence for improved intracellular targeting. The myristoyl chain enhances membrane association, while basic residues facilitate translocation. Researchers study its interaction with cytoskeletal proteins and signaling complexes. Applications include delivery-peptide engineering, intracellular targeting, and protein-protein interaction modulation.
CAT No: R2771
Myr-tat-CBD3 is a synthetic peptide conjugate designed for advanced research applications in cellular signaling and protein interaction studies. Structurally, it combines a myristoyl group and a tat peptide sequence with the CBD3 domain, facilitating membrane association and intracellular delivery. The unique configuration of Myr-tat-CBD3 enables selective modulation of protein-protein interactions involving the collapsin response mediator protein 2 (CRMP2), a key regulator in neuronal development and axonal guidance. Its ability to penetrate cell membranes and target specific intracellular pathways makes it a valuable tool for dissecting molecular mechanisms underlying cytoskeletal dynamics and signal transduction in experimental systems.
Cellular trafficking research: Myr-tat-CBD3 is widely utilized to investigate the intracellular trafficking of signaling proteins, particularly those associated with the cytoskeleton. By mimicking or disrupting native interactions between CRMP2 and its binding partners, the peptide provides insights into the spatial and temporal regulation of protein localization within cells. Researchers employ it to elucidate how alterations in protein trafficking contribute to cellular morphology and function, enabling a deeper understanding of processes such as neurite outgrowth and synaptic plasticity.
Protein interaction modulation: The peptide's design allows it to selectively interfere with CRMP2-mediated protein complexes, serving as a powerful probe for mapping interaction networks. In vitro and cell-based assays leverage Myr-tat-CBD3 to delineate the functional consequences of disrupting specific protein-protein interfaces. This application is particularly valuable in studies aiming to characterize the roles of CRMP2 in cytoskeletal rearrangement, vesicle transport, and intracellular signaling cascades.
Neuronal signaling pathway analysis: Due to its targeted action on CRMP2, Myr-tat-CBD3 is frequently used in the analysis of neuronal signaling pathways that govern axonal growth and guidance. Experimental models benefit from its capacity to modulate downstream effectors, allowing for precise dissection of the molecular events that regulate neural network formation. Such studies contribute to the broader understanding of neurodevelopmental processes and the molecular basis of synaptic connectivity.
Peptide delivery and uptake studies: The inclusion of the tat sequence in Myr-tat-CBD3 enhances its cell-penetrating properties, making it an effective tool for assessing peptide delivery mechanisms. Researchers utilize it to optimize protocols for intracellular delivery of functional peptides and to evaluate the efficiency of cell-penetrating sequences in various cell types. These investigations inform the design of next-generation delivery vectors for research applications involving intracellular targeting.
Functional dissection of cytoskeletal regulation: Myr-tat-CBD3 serves as a specialized reagent for probing the regulation of microtubule dynamics and actin organization. By modulating CRMP2 activity, the peptide enables controlled perturbation of cytoskeletal assembly and stability in experimental settings. This application supports the identification of regulatory checkpoints in cytoskeletal remodeling, facilitating the development of new hypotheses regarding the coordination of cell shape, motility, and intracellular transport in both neuronal and non-neuronal systems.
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