N6-L-Lysyl-L-Lysine features a lysine-lysine dimer connected through the ε-amino group, generating a branched, highly basic structure. Multiple protonatable sites support polyamine-mimetic interactions with nucleic acids and acidic proteins. Researchers use it to study charge density, condensation phenomena, and cross-linking chemistry. Applications include protein-modification research, chromatin-modeling studies, and polycation design.
CAT No: R2720
CAS No:94613-75-9
Synonyms/Alias:N6-L-Lysyl-L-lysine;N~6~-L-Lysyl-L-Lysine;L-Lysine, N6-L-lysyl-;CQ27MY2BS0;94613-75-9;UNII-CQ27MY2BS0;Q27453403;
N6-L-Lysyl-L-lysine is a synthetic dipeptide composed of two L-lysine residues joined via an amide bond at the N6 position of the side chain. As a structurally defined peptide compound, it serves as a valuable tool in biochemical and molecular research, particularly in studies involving protein modification, peptide-protein interactions, and enzymatic specificity. Its unique configuration provides a model for exploring the roles of lysine-rich motifs in biological systems, and it is frequently utilized to advance understanding in the fields of enzymology, structural biology, and peptide chemistry.
Peptide substrate studies: N6-L-Lysyl-L-lysine is widely employed as a model substrate in assays investigating the activity and specificity of lysine-modifying enzymes, such as transglutaminases, lysine methyltransferases, and acetyltransferases. By providing a defined dipeptide structure with an accessible lysine side chain, it enables researchers to probe the catalytic mechanisms, substrate preferences, and kinetic properties of these enzymes. This facilitates the characterization of enzyme function and the elucidation of post-translational modification pathways involving lysine residues.
Enzyme inhibition and screening: The compound is instrumental in the development and optimization of biochemical assays designed to identify and characterize inhibitors of lysine-targeting enzymes. Its defined structure and reactivity allow for the establishment of reproducible assay conditions, supporting high-throughput screening efforts and mechanistic studies. Researchers leverage its properties to assess inhibitor potency, selectivity, and mode of action, thereby aiding in the advancement of enzyme-targeted research and drug discovery initiatives.
Protein crosslinking research: N6-L-Lysyl-L-lysine serves as a model system for studying protein crosslinking reactions, particularly those mediated by transglutaminases and related enzymes. Its bifunctional lysine moieties provide reactive sites for the formation of isopeptide bonds, enabling detailed investigations into the chemistry and biological consequences of protein crosslinking. Such studies are critical for understanding protein network formation, structural stabilization, and the regulation of protein-protein interactions in cellular and extracellular environments.
Peptide synthesis and analytical method development: The dipeptide is utilized as a reference standard and building block in peptide synthesis protocols, as well as in the development and validation of analytical techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry. Its well-characterized structure facilitates method calibration, system suitability testing, and the evaluation of peptide modification strategies. This supports the accurate quantification and identification of lysine-containing peptides in complex mixtures, enhancing the reliability of peptide analysis workflows.
Structural biology and conformational studies: Researchers employ N6-L-Lysyl-L-lysine to investigate the conformational properties of lysine-rich peptides and their influence on higher-order protein structure. By serving as a model for lysine-lysine linkages, it aids in the exploration of side chain interactions, hydrogen bonding patterns, and the impact of specific modifications on peptide folding. Insights gained from such studies contribute to a deeper understanding of protein architecture, stability, and function, informing the rational design of biomolecules with tailored properties.
1. Emu oil in combination with other active ingredients for treating skin imperfections
2. Cell-based adhesion assays for isolation of snake venom’s integrin antagonists
4. Immune responses to homocitrulline-and citrulline-containing peptides in rheumatoid arthritis
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More