PGLU-HIS-PRO Amide Acetate Salt is a peptide-based compound composed of the amino acids Proline, Glycine, Leucine, Histidine, and Proline. It is a salt form of the amide acetate, which is a derivative of acetic acid. This compound is used in research and development for various applications, including drug delivery, protein engineering, and bioconjugation. It is also used in the production of peptide-based drugs and other pharmaceuticals.
CAT No: 10-101-209
CAS No:40216-95-3
Synonyms/Alias:Thyroliberin acetate salt;40216-95-3;acetic acid;(2S)-N-[(2S)-1-[(2S)-2-carbamoylpyrrolidin-1-yl]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]-5-oxopyrrolidine-2-carboxamide;TRF Acetate;PGLU-HIS-PRO AMIDE ACETATE SALT;SCHEMBL4406163;DTXSID00739560;AKOS030485975;AKOS040759053;TS-09569;Protirelin Acetate(24305-27-9 free base);F85399;Acetic acid--5-oxo-L-prolyl-L-histidyl-L-prolinamide (1/1);(S)-N-((S)-1-((S)-2-carbamoylpyrrolidin-1-yl)-3-(1H-imidazol-4-yl)-1-oxopropan-2-yl)-5-oxopyrrolidine-2-carboxamide acetate;
PGLU-HIS-PRO Amide Acetate Salt is a synthetic tripeptide composed of pyroglutamyl, histidyl, and prolyl residues, presented in its amidated and acetate salt form. As a structurally defined peptide, it is valued in biochemical research for its potential to model peptide-protein interactions, serve as a substrate or ligand in enzymatic assays, and facilitate studies on peptide stability and receptor binding. The presence of the pyroglutamyl residue at the N-terminus and the amide modification at the C-terminus confer unique physicochemical properties, making this tripeptide a versatile tool for peptide-focused investigations. Its precise sequence and modifications enable researchers to explore structure-activity relationships and post-translational modifications relevant to bioactive peptides in cellular signaling and regulatory processes.
Peptide-Protein Interaction Studies: Researchers frequently utilize PGLU-HIS-PRO Amide Acetate Salt to investigate the molecular basis of peptide recognition by proteins, including receptors and enzymes. The defined tripeptide sequence, featuring the N-terminal pyroglutamyl group, is particularly useful for probing the impact of N-terminal modifications on binding affinity and specificity. By incorporating this tripeptide into binding assays or co-crystallization experiments, scientists can elucidate the structural determinants that govern peptide-mediated molecular recognition events, ultimately advancing understanding of key biological pathways.
Enzymatic Substrate Analysis: The tripeptide's composition and modifications make it an effective substrate for peptidase and protease activity assays. Its resistance or susceptibility to enzymatic cleavage provides insight into enzyme specificity, substrate recognition motifs, and the influence of post-translational modifications such as N-terminal cyclization and C-terminal amidation. By monitoring the hydrolysis or stability of this peptide in the presence of various proteolytic enzymes, investigators can characterize enzymatic mechanisms and identify potential inhibitors or modulators.
Peptide Stability and Degradation Research: The stability of synthetic peptides under physiological and experimental conditions is a critical consideration in peptide-based research. PGLU-HIS-PRO Amide Acetate Salt, with its terminal modifications, serves as a model compound for examining the effects of pyroglutamylation and amidation on peptide degradation pathways. Studies employing this tripeptide help clarify how structural alterations influence resistance to exopeptidases and overall peptide half-life, supporting the rational design of more stable peptide analogs for research applications.
Receptor Binding and Signal Transduction Investigations: The unique sequence and modifications present in this tripeptide provide a platform for studying ligand-receptor interactions, particularly in systems where N-terminally modified peptides play a regulatory role. By using the compound in receptor binding assays or cell-based signaling studies, researchers can dissect the contribution of specific sequence motifs to receptor activation, desensitization, or antagonism. These insights are valuable for mapping signaling cascades and identifying critical points of regulation in peptide-mediated communication.
Peptide Synthesis and Method Development: PGLU-HIS-PRO Amide Acetate Salt is also employed as a reference standard or intermediate in the optimization of solid-phase peptide synthesis protocols. Its well-defined structure and functional group diversity make it suitable for validating coupling strategies, deprotection steps, and purification techniques. By serving as a model peptide, it supports the development of robust synthetic methodologies and quality control procedures, facilitating the efficient production of custom peptides for diverse research needs.
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