Polymyxin B2 Sulfate presents a cyclic-linear lipopeptide architecture enriched with basic residues that support lipid-binding studies. Its amphipathic character drives solvent-dependent conformational changes. Researchers examine charge-driven interactions with membrane mimetics. Applications include structural biology, peptide-material exploration, and sequence-activity correlation.
CAT No: R2718
CAS No:108965-69-1
Synonyms/Alias:UNII-WTC2W6JDTU;WTC2W6JDTU;108965-69-1;Polymyxin B2 (sulfate);Q27292833;N-[(2S)-4-amino-1-[[(2S,3R)-1-[[(2S)-4-amino-1-oxo-1-[[(3S,6S,9S,12S,15R,18S,21S)-6,9,18-tris(2-aminoethyl)-15-benzyl-3-[(1R)-1-hydroxyethyl]-12-(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxobutan-2-yl]-6-methylheptanamide;sulfuric acid;
Polymyxin B2 Sulfate is a cationic cyclic lipopeptide antibiotic derived from the bacterium *Bacillus polymyxa*. As a member of the polymyxin family, it is characterized by a unique amphipathic structure that enables high-affinity interactions with the outer membrane of Gram-negative bacteria. Its primary mode of action involves disruption of bacterial cell membrane integrity, making it a widely studied agent in microbiological and biochemical research. Polymyxin B2 Sulfate is valued for its specificity toward lipopolysaccharide-rich membranes and is frequently used as a reference compound in studies of antimicrobial activity, membrane permeability, and bacterial resistance mechanisms.
Antimicrobial susceptibility testing: Researchers commonly employ Polymyxin B2 Sulfate in laboratory protocols to assess the sensitivity of Gram-negative bacterial strains to lipopeptide antibiotics. By incorporating defined concentrations into broth or agar-based assays, investigators can determine minimum inhibitory concentrations (MICs) and analyze resistance profiles in clinical and environmental isolates. Its well-characterized mechanism of targeting lipid A in lipopolysaccharides makes it a reliable standard for benchmarking antimicrobial efficacy and studying the emergence of polymyxin resistance.
Membrane permeability studies: The compound's ability to disrupt bacterial outer membranes underpins its use in probing membrane integrity and permeability. Experimental systems utilize Polymyxin B2 Sulfate to induce controlled membrane perturbations, allowing scientists to investigate the physicochemical properties of bacterial envelopes, characterize transport processes, and evaluate the efficacy of novel membrane-targeting agents. Its defined interaction with phospholipids and lipopolysaccharides supports mechanistic studies into the structure-function relationships governing bacterial defense.
Endotoxin neutralization assays: Due to its strong binding affinity for lipid A moieties in bacterial endotoxins, Polymyxin B2 Sulfate is frequently incorporated into in vitro assays designed to neutralize or quantify endotoxin activity. In biochemical and cell-based studies, it serves as a tool for sequestering lipopolysaccharides, thereby enabling researchers to dissect inflammatory signaling pathways, validate endotoxin removal protocols, or assess the biological impact of endotoxin contamination in experimental systems.
Bacterial resistance mechanism research: The ongoing emergence of multidrug-resistant Gram-negative pathogens has intensified interest in the molecular basis of polymyxin resistance. Polymyxin B2 Sulfate is routinely used to challenge mutant or engineered bacterial strains, facilitating the elucidation of adaptive modifications such as altered lipid A acylation, efflux pump expression, or surface charge changes. These studies contribute to a deeper understanding of resistance evolution and inform the development of next-generation antimicrobial strategies.
Analytical method development: In analytical chemistry and quality control settings, Polymyxin B2 Sulfate acts as a reference standard for the quantification and detection of polymyxin-class antibiotics. Its defined chemical properties enable the calibration of chromatographic and spectrometric methods, supporting the accurate measurement of related compounds in pharmaceutical formulations, fermentation broths, or biological samples. This application is essential for ensuring the reliability and consistency of analytical workflows in research and industrial laboratories.
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More