Rad17 Derived Peptide represents a segment of the Rad17 protein used to investigate checkpoint signaling and protein-protein interfaces. Polar, charged, and hydrophobic residues contribute to structured recognition. Researchers employ it in binding assays and phosphorylation-related studies. Applications include DNA-damage pathway research, motif-function analysis, and structural modeling.
CAT No: R2824
Synonyms/Alias:Rad17 derived peptide; SP-050; SP050; ASELPASQPQPFSAKKK
Rad17 derived peptide is a synthetic fragment modeled after the Rad17 protein, a key component of the DNA damage response pathway. This peptide is engineered to mimic functional regions of the native Rad17, enabling researchers to study its specific roles in cellular processes such as checkpoint activation, DNA repair, and cell cycle regulation. As a tool for molecular biology and biochemistry, the peptide offers high specificity for interacting with proteins involved in the DNA damage recognition and signaling cascade. Its utility extends to dissecting protein-protein interactions, mapping phosphorylation events, and exploring the molecular underpinnings of genome stability. Due to its defined sequence and customizable modifications, Rad17 derived peptide serves as a versatile reagent for in vitro and in vivo studies, supporting a wide range of experimental approaches in the fields of cell biology and genetics.
DNA Damage Response Research: In the context of DNA damage response research, Rad17 derived peptide is utilized to elucidate the mechanisms by which cells detect and respond to genotoxic stress. By introducing the peptide into cellular or cell-free systems, scientists can observe its impact on the recruitment and activation of checkpoint proteins such as ATR, the 9-1-1 complex, and other mediators of the DNA repair pathway. This approach enables detailed mapping of the Rad17-dependent signaling axis, allowing for the identification of critical interaction sites and post-translational modifications that govern checkpoint activation. The peptide's ability to competitively inhibit or mimic native Rad17 interactions makes it a valuable tool for dissecting the sequence of molecular events following DNA damage.
Protein-Protein Interaction Studies: For protein-protein interaction studies, the Rad17 derived peptide serves as an affinity probe to identify and characterize binding partners of the Rad17 protein. Researchers employ the peptide in pull-down assays, co-immunoprecipitation, and surface plasmon resonance experiments to investigate how Rad17 interfaces with other components of the checkpoint machinery. By analyzing the binding kinetics and specificity of these interactions, scientists gain insights into the structural determinants of Rad17 function and the assembly of multi-protein complexes involved in genome surveillance. This knowledge can inform the rational design of modulators targeting the DNA damage checkpoint for research applications.
Phosphorylation and Post-Translational Modification Analysis: In studies focused on phosphorylation and post-translational modifications, Rad17 derived peptide is used as a substrate to assess the activity of kinases such as ATR and ATM. By incorporating specific phosphorylation sites, the peptide allows for quantitative analysis of kinase-substrate relationships, enabling researchers to monitor dynamic changes in phosphorylation status in response to DNA damage. This application is instrumental in identifying novel regulatory mechanisms that control Rad17 activity and checkpoint signaling, contributing to a deeper understanding of cellular adaptation to genomic insults.
Cell Cycle Regulation Investigations: Within cell cycle regulation investigations, the peptide provides a means to probe the role of Rad17 in the transition between cell cycle phases. By modulating Rad17-mediated signaling pathways, researchers can assess the impact on G2/M checkpoint enforcement, replication fork stability, and the prevention of premature mitotic entry. The peptide's targeted action facilitates the dissection of Rad17's contribution to maintaining cell cycle fidelity, supporting research into the prevention of chromosomal instability and aneuploidy.
Synthetic Biology and Tool Development: In the sphere of synthetic biology and tool development, Rad17 derived peptide is harnessed as a modular component for constructing biosensors and engineered signaling networks. Its well-characterized interaction motifs and post-translational modification sites enable integration into synthetic circuits that monitor or modulate DNA damage responses. Scientists leverage these properties to develop innovative research tools for high-throughput screening, pathway engineering, and the creation of customizable models for genome integrity studies. Through these diverse applications, Rad17 derived peptide stands as a pivotal resource for advancing molecular and cellular research into genome maintenance mechanisms.
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