Sulfo-Cyanine5.5-Tyramide is a highly water-soluble fluorescent tyramide bearing an activated aromatic moiety for enzymatic deposition studies. The sulfonated dye provides bright near-infrared fluorescence and strong hydrophilicity. Researchers utilize it for signal amplification and spatial mapping. Applications include biochemical labeling, imaging workflows, and substrate-chemistry analysis.
CAT No: R2844
Sulfo-Cyanine5.5-Tyramide is a highly water-soluble fluorescent labeling reagent designed for advanced biochemical and molecular biology applications. Featuring a sulfonated Cyanine5.5 fluorophore conjugated to a tyramide moiety, this compound is optimized for use in signal amplification systems, enabling researchers to achieve superior sensitivity and specificity in detection assays. The unique structure of Sulfo-Cyanine5.5-Tyramide allows for covalent labeling in the presence of peroxidase enzymes, making it a powerful tool for visualizing low-abundance targets in complex biological samples. Its far-red fluorescence emission profile minimizes background interference and autofluorescence, thus providing clear, high-contrast imaging in both cell and tissue contexts. The hydrophilic nature of the sulfo group ensures excellent solubility in aqueous buffers, enhancing compatibility with a wide range of experimental conditions and reducing nonspecific binding.
Immunohistochemistry Signal Amplification: Sulfo-Cyanine5.5-Tyramide is widely employed in tyramide signal amplification (TSA) protocols for immunohistochemistry (IHC). In these applications, the compound serves as a substrate for horseradish peroxidase (HRP)-conjugated antibodies. Upon enzymatic activation, the tyramide moiety is converted to a highly reactive intermediate that covalently binds to tyrosine residues in the vicinity of the enzyme. This results in robust and localized deposition of the Cyanine5.5 fluorophore, significantly enhancing the detection of low-abundance antigens in tissue sections. Researchers benefit from increased sensitivity and spatial resolution, allowing for the visualization of subtle expression patterns that may otherwise be undetectable with conventional fluorescent labeling techniques.
In Situ Hybridization Detection: The reagent is also integral to in situ hybridization (ISH) workflows, where it enables the sensitive detection of specific nucleic acid sequences within cells and tissues. By leveraging the HRP-catalyzed deposition of the fluorescent tyramide, Sulfo-Cyanine5.5-Tyramide permits the visualization of RNA or DNA targets with exceptional clarity. This approach is particularly valuable in studies of gene expression, viral infection, or chromosomal localization, as it allows for precise mapping of nucleic acids in their native cellular context. The far-red emission of Cyanine5.5 further facilitates multiplexing with other fluorophores for comprehensive molecular profiling.
Multiplexed Fluorescent Imaging: The unique spectral properties of the Cyanine5.5 dye make this compound ideal for multiplexed imaging applications. Researchers can combine Sulfo-Cyanine5.5-Tyramide with other tyramide-based fluorophores in parallel staining procedures, enabling the simultaneous detection of multiple targets within a single sample. This capability is especially valuable for complex studies involving co-localization, cellular phenotyping, or spatial transcriptomics, where distinguishing between several markers in the same tissue section is critical. The compound's high photostability and minimal spectral overlap with commonly used dyes further enhance its utility in advanced imaging workflows.
Western Blot Signal Enhancement: In protein analysis, Sulfo-Cyanine5.5-Tyramide can be utilized to amplify signals in western blot assays. By incorporating it into HRP-based detection systems, researchers achieve greater sensitivity in identifying low-abundance proteins on membranes. The covalent deposition mechanism ensures that the fluorescent signal remains stable during subsequent washes and imaging steps, providing reliable and reproducible results. This approach is particularly advantageous when working with scarce samples or when quantifying subtle changes in protein expression.
Cellular Pathway Analysis: Beyond its role in detection assays, Sulfo-Cyanine5.5-Tyramide supports the study of cellular signaling pathways by enabling the visualization of post-translational modifications or protein-protein interactions. When used in proximity ligation assays or other enzyme-mediated labeling techniques, the compound facilitates the mapping of dynamic molecular events within intact cells. The high sensitivity and specificity of tyramide-based amplification allow for the detection of transient or low-abundance modifications that are critical for understanding complex biological processes. Researchers gain valuable insights into cellular mechanisms, supporting advancements in fundamental biology and translational research.
2. Adipose tissue is a key organ for the beneficial effects of GLP-2 metabolic function
4. SERS spectrum of the peptide thymosin‐β4 obtained with Ag nanorod substrate
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