Sulfo-Cyanine5.5-Tyramide is a highly water-soluble fluorescent tyramide bearing an activated aromatic moiety for enzymatic deposition studies. The sulfonated dye provides bright near-infrared fluorescence and strong hydrophilicity. Researchers utilize it for signal amplification and spatial mapping. Applications include biochemical labeling, imaging workflows, and substrate-chemistry analysis.
CAT No: R2844
Sulfo-Cyanine5.5-Tyramide is a water-soluble fluorescent labeling reagent that combines the high-performance Sulfo-Cyanine5.5 dye with a tyramide moiety, enabling robust signal amplification in biochemical assays. As a member of the cyanine dye family, it offers strong near-infrared fluorescence and excellent photostability, making it particularly valuable for sensitive detection methods. The sulfonated structure enhances aqueous solubility and minimizes nonspecific binding, which is critical for applications in complex biological samples. Its unique tyramide conjugation allows for enzyme-mediated covalent deposition, facilitating highly localized and stable labeling for advanced imaging and analytical workflows. Researchers in molecular biology, biochemistry, and cell biology rely on this reagent for its specificity, intensity, and compatibility with a variety of experimental platforms.
Signal amplification: In proximity-based detection systems, Sulfo-Cyanine5.5-Tyramide is widely used in tyramide signal amplification (TSA) protocols. The tyramide group serves as a substrate for horseradish peroxidase (HRP), which catalyzes the deposition of the fluorescent label onto tyrosine residues of proteins at the site of enzymatic activity. This covalent labeling process dramatically increases the sensitivity of immunohistochemistry (IHC), in situ hybridization (ISH), and western blotting, allowing researchers to detect low-abundance targets that might otherwise be undetectable.
Multiplex fluorescence imaging: The distinctive spectral properties of the Sulfo-Cyanine5.5 dye, with emission in the near-infrared range, enable its integration into multiplexed imaging experiments. By using this reagent in combination with other spectrally distinct tyramide conjugates, scientists can simultaneously visualize multiple molecular targets within the same biological specimen. This facilitates complex studies of cellular signaling networks, tissue architecture, and biomarker co-localization in both fixed and fresh samples.
Cellular and tissue labeling: The high water solubility and low nonspecific background of this fluorescent tyramide make it exceptionally well-suited for labeling cells and tissues in challenging environments. Its ability to produce stable, covalent fluorescent deposits ensures that signals remain robust during extended imaging sessions or downstream processing. Researchers employ this reagent to achieve precise spatial resolution in studies of protein distribution, cellular morphology, and tissue microenvironments.
Quantitative bioanalytical assays: The intense and stable fluorescence of Sulfo-Cyanine5.5-Tyramide supports its use in quantitative bioanalytical applications, such as microplate-based immunoassays and flow cytometry. Its compatibility with HRP-mediated detection systems allows for accurate quantification of target analytes, even at low concentrations. The reagent's performance in these assays contributes to improved assay sensitivity and reproducibility, supporting high-throughput screening and detailed kinetic analyses.
Advanced molecular diagnostics research: In the development of novel molecular diagnostic tools, this sulfonated cyanine dye-tyramide conjugate is leveraged for its superior signal-to-noise characteristics and adaptability to various assay formats. Its use in research-stage protocols—including proximity ligation assays, RNA detection, and protein-protein interaction studies—enables the generation of high-resolution data critical for biomarker discovery and validation. The reagent's robust performance and versatility make it a valuable component in the toolkit of scientists advancing the frontiers of molecular analysis.
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