Kisspeptin is the new favorite tool of the endocrinologist and neuroscientist. Arguably more than any other pharmacological probe, kisspeptin, as the upstream-most signaling molecule of the reproductive axis, represents the 'master key' to turning the hypothalamic-pituitary-gonadal axis (HPG) on or off, respectively, without the less selective polypharmacology associated with the long-used gonadotropin releasing hormone (GnRH) or gonadotropin (GtH) secretagogues. In addition, the peptide's minimal length, receptor selectivity, and fast kinetics provide the experimental flexibility to acutely drive a peak in luteinizing hormone (LH) secretion to simulate a pre-ovulatory surge, or alternatively low-frequency pulsatile stimulation to allow follicle-stimulating hormone (FSH) to predominate, or to remove kisspeptin to collapse the axis, in the same preparation. These experimental capabilities are critically important when the nature of the question requires resolution of time codes, steroid feedback effects, and/or metabolic gating, which cannot be interrogated with steady state hormone level measurements.
The neuropeptide kisspeptin is the product of the KISS1 gene. It is considered to be a pivotal regulator of the HPG axis. Increasingly, kisspeptin is finding its way into the laboratory setting due to its wide-ranging physiological significance. As a laboratory-grade reagent, kisspeptin is now becoming a versatile tool for research in the fields of reproductive biology, neuroendocrine signaling, and even cancer biology. Kisspeptin provides a chemically defined reagent, as opposed to a highly complex and potentially uncharacterized endogenous hormone preparation. Its extreme evolutionary conservation across mammals, in addition to its receptor Kiss1R, also being well characterized, places kisspeptin in an enviable position about translatability from rodent models of reproductive pathophysiology to humans. Furthermore, the batches of synthetically generated kisspeptin that have been verified to have high purity are now able to generate a high level of reproducibility in modern in-vitro and in-vivo studies, where the resultant effects are the direct action of this exclusive ligand–receptor pair without the complicating nature of potential contaminants and degradation products. Research laboratories investigating the mechanisms behind puberty initiation and fertility or metabolic–reproductive interactions require a quality product of kisspeptin to achieve an interpretable mechanistic understanding.
Fig. 1 In vitro actions of kisspeptin (Kp). 1,2
Kisspeptin is the endogenous, and most upstream, excitatory drive that directly stimulates GnRH neurons, so it can be used to experimentally de-orphanise them, turning them from the quiescent state they are in during childhood to the pulsatile state of adult reproductive life. For example, when kisspeptin is applied exogenously to hypothalamic slices, it elicits strong, pulsatile release from GnRH terminals, and this can be used to study the ion-channel dynamics responsible for peptide-induced GnRH burst firing, in the absence of any steroid feedback. Systemic or central administration of kisspeptin in intact animals mimics the pre-ovulatory LH surge, and so, as an ethically permissible proxy, it can be used to study oocyte maturation, CL competence, and luteal-phase adequacy in the face of genetic or dietary perturbations. Continuous kisspeptin infusion can be used to study the timing of puberty, as it can accelerate vaginal opening and/or testicular descent in juvenile animals, and so clarify the way in which environmental information (photoperiod, energy density, etc.) is transduced into neuroendocrine signals. Kisspeptin is also useful in studies of sex-steroid feedback, since its own expression is up-regulated by oestradiol (female) or down-regulated by androgens (male); conversely, selective knockdown of kisspeptin can be used to distinguish positive from negative feedback, without the need to ablate the gonads. Finally, the presence of a C-terminal RF-amide motif, which is conserved and resistant to DPP-IV in many kisspeptins (including the mouse and human kisspeptin-1), gives the peptides greater stability in the CSF compared with native GnRH. This makes them more amenable to microdialysis and push–pull perfusion experiments for quantification of peptide release.
Discovered as a metastasis-suppressor gene product, kisspeptin also remains of interest to cancer biologists given that its receptor is found on several extra-hypothalamic tissues (e.g., placental trophoblasts, pancreatic islets, adipose stroma). Treatment with kisspeptin in cell-culture models decreases motility and anchorage-independent growth of carcinoma cell lines, providing a specific tool to parse out the autocrine loops that drive epithelial–mesenchymal transition (EMT) without using crude tissue extracts. The peptide also attenuates angiogenic sprouting in ex-vivo aortic ring assays, allowing the dissection of paracrine signalling between endothelial cells and perivascular adipose tissues under defined growth-factor conditions. Neuroendocrine approaches have been used to demonstrate that kisspeptin neurons co-express neurokinin B and dynorphin, the so-called KNDy system that underlies GnRH pulsatility; as such, selective kisspeptin antagonists or agonists are switch-tools for studying how the interaction between three neuropeptides can encode frequency information that preferentially promotes LH versus FSH secretion. The observation that the peptide's action also integrates metabolic signals (leptin, insulin, glucose) has made kisspeptin a candidate system for coupling obesity or diabetes with reproductive dysfunction, an emerging field where kisspeptin infusions are used to determine whether restoration of central kisspeptin tone can ameliorate ovarian anovulation or testicular hypotrophy imposed by high-fat feeding or insulin-receptor antagonism.
Researchers using synthetic kisspeptin at a laboratory scale are expanding its applications beyond traditional uses to investigate different behavioral responses and cardiovascular functions. Administration of kisspeptin via micro-injection into the cerebroventricular system during open-field behavior or partner-preference paradigms has been shown to cause a dose-dependent increase in exploratory or mounting behavior. This pharmacological approach is useful for teasing out neural pathways involved in the central processing of steroid signals into motivational states that are independent of the more widespread peripheral effects of systemic steroids. Arterial telemetry has also demonstrated that kisspeptin increases sympathetic tone and affects baroreflex sensitivity, providing a robust experimental manipulation for studying central-peripheral coupling in freely moving animals. As a whole, the diverse utility of kisspeptin as a tool in one reagent that can be applied to studies of reproductive physiology as well as metabolism, cancer, and cardiovascular health makes it a very useful molecule to have available in a laboratory in order to reduce cost and increase experimental return on investment.
Orthogonal assay, tamper-evident packaging and live temperature monitoring provide an audit trail from synthesis through freeze/thaw to create a quality-assured reagent that is fully traceable and stability validated, rather than just a reagent. The quality-dossier is living: high resolution mass spec, dual pH chromatograms and cell-based bioassays for fingerprinting. Oxygen/moisture/light barrier in metallized pouches, siliconized amber glass ampoules and fractured-hologram seals provide tamper evidence and containment. Every cold-chain shipment has its temperature recorded on an encrypted data-logger that will flag any excursions and automatically trigger a replacement shipment if necessary, so that what arrives in the lab is what left, an anhydrous, sterile, fully identity confirmed, traceable and stability validated reagent ready to produce reproducible science.
Each research-quality batch is processed through an analytical cascade that should leave no doubt about the molecular identity or purity. The initial confirmatory test is high-resolution mass spectrometry in both positive- and negative-ion modes, such that the intact molecular ion and its isotopic envelope can be compared to the theoretical monoisotopic mass calculated from the elemental composition. Acquisition of the collision-induced fragmentation spectra can also be done in parallel to further confirm sequence integrity by comparing each product ion to the expected cleavages along the peptide backbone. Reversed-phase chromatography is then performed under two different pH gradients so that hydrophobic contaminants, which often result from side-chain oxidation and/or dityrosine cross-linking, can be separated from the parent molecule even when the retention times only differ by a few seconds. Area-percent integration is completed with algorithmic peak deconvolution to reduce manual bias, and the resulting electropherograms are saved electronically so that any subsequent analyses can re-interrogate the raw data files without re-analysis.
To the maximum extent possible, exposure to moisture, oxygen, and photons has been treated equally by the packaging approach. Lyophilized cake adhering to the inside of the siliconized amber borosilicate vial is minimized by electrostatic repulsion, and the vial is stoppered with a fluoropolymer-faced closure inserted under light vacuum. An aluminium overseal imprinted with a tamper-evident hologram, which irreversibly cracks when any attempt at lifting is made, is then applied, providing visual assurance of integrity. The primary container is placed in a metallized pouch with oxygen transmission rate near zero, and a food grade desiccant packet is included to control humidity to a level that is known to be conducive to the peptide's stability over extended periods.
Our catalog includes high-purity Kisspeptin-10 and Kisspeptin-54 peptides specifically designed for laboratory research. Each batch is manufactured under strict quality control with HPLC and MS validation, ensuring reliable results for fertility, oncology, and neuroendocrine studies. If your research requires a tailored solution, we also provide custom peptide synthesis services. From modified sequences to bulk supply, our team can deliver Kisspeptin peptides customized to your project's needs while maintaining reproducibility and quality assurance.
Advance your projects with premium Kisspeptin peptides trusted by researchers worldwide. Place your order online today or reach out to discuss bulk pricing, custom synthesis options, or technical support. We're committed to delivering high-quality peptides with fast, secure global shipping to meet your lab's timeline.
Kisspeptin Peptides We Provides
| CAT# | Product Name | M.W | Molecular Formula | Inquiry |
|---|---|---|---|---|
| K04001 | Kisspeptin-13 (4-13) (human) | 1302.46 | C63H83N17O14 | Inquiry |
| K04002 | Kisspeptin-54 (human) | 5857.51 | C258H401N79O78 | Inquiry |
| K04003 | Kisspeptin-54 (27-54) (human) | 3229.69 | C149H226N42O39 | Inquiry |
| K04004 | Kisspeptin-13 (human) | 1626.84 | C78H107N21O18 | Inquiry |
| M04006 | Kisspeptin-10 Metastin (45-54), Human | C63H83N17O14 | Inquiry | |
| M04007 | Kisspeptin-13 | C78H107N21O18 | Inquiry | |
| M13002 | Kisspeptin-14 | Inquiry | ||
| M13006 | Kisspeptin-10_mouse | Inquiry | ||
| R0925 | Kisspeptin 10 (dog) | 1330.51 | C65H87N17O14 | Inquiry |
| R0938 | Kisspeptin 234 | 1295.4 | C63H78N18O13 | Inquiry |
| R1469 | Kisspeptin-10 | 1302.4 | C63H83N17O14 | Inquiry |
| R1470 | Kisspeptin-10 Trifluoroacetate | 1416.46 | C63H83N17O14.C2HF3O2 | Inquiry |
| R2281 | Kisspeptin-10, rat | 1318.4 | C63H83N17O15 | Inquiry |
| R2438 | Kisspeptins | 5857 | C258H401N79O78 | Inquiry |
| R2372 | Kisspeptin-54 (27-54) (human) trifluoroacetate salt | 3229.6 | C149H226N42O39 | Inquiry |
1. Are these Kisspeptin peptides for clinical use?
No. Our products are intended strictly for laboratory research only and are not approved for human or veterinary applications.
2. What formats are available for Kisspeptin peptides?
We offer Kisspeptin-10 and Kisspeptin-54 in lyophilized powder form, available in multiple pack sizes for flexibility in experimental design.
3. Can I request Kisspeptin in bulk quantities?
Yes. We provide bulk supply options for research institutions and pharmaceutical companies, ensuring consistent quality across large projects.
4. Do you provide quality documentation?
Every peptide comes with a certificate of analysis (CoA) including HPLC and MS data for transparency and reliability.
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