Ac-IEPD-AFC (IEPD) is a substrate of Granzyme B.
CAT No: R1148
CAS No:1135417-31-0
Synonyms/Alias:Ac-IEPD-AFC;1135417-31-0;N-Acetyl-Ile-Glu-Pro-Asp-7-amino-4-trifluoromethylcoumarin;4-[(2-acetamido-3-methylpentanoyl)amino]-5-[2-[[3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]carbamoyl]pyrrolidin-1-yl]-5-oxopentanoic acid;Ac-DL-xiIle-DL-Glu-DL-Pro-DL-Asp-Unk;DA-60766;PD118626;
Ac-IEPD-AFC is a synthetic peptide substrate widely utilized in biochemical research for the specific detection and quantification of caspase-8 activity. Structurally, it features an acetylated tetrapeptide sequence (IEPD) linked to the fluorogenic reporter group 7-amino-4-trifluoromethylcoumarin (AFC). This design enables sensitive monitoring of proteolytic cleavage events, particularly those mediated by caspase-8 and related proteases. Its high specificity and robust fluorescence signal upon enzymatic cleavage make it an indispensable reagent for studies investigating apoptosis, protease kinetics, and inhibitor screening in cell-free and cellular systems.
Enzyme Activity Assays: The substrate is extensively used for measuring caspase-8 enzymatic activity in vitro. Upon cleavage at the IEPD recognition site by active caspase-8, the AFC fluorophore is released, producing a quantifiable fluorescence signal. This property allows researchers to assess caspase-8 activation dynamics in response to various stimuli or experimental conditions, facilitating detailed characterization of apoptotic signaling pathways.
Apoptosis Research: Ac-IEPD-AFC plays a critical role in apoptosis studies by enabling real-time detection of caspase-8 activation, a key event in the extrinsic apoptotic pathway. Its application in cell lysates or purified enzyme preparations supports the investigation of death receptor-mediated signaling, providing insight into the molecular mechanisms governing programmed cell death and its regulation by upstream modulators.
Inhibitor Screening: The fluorogenic substrate is routinely employed in high-throughput screening platforms for the identification and characterization of caspase-8 inhibitors. By monitoring changes in fluorescence intensity, researchers can evaluate the potency and selectivity of candidate compounds, advancing the development of novel modulators targeting apoptotic proteases for research purposes.
Protease Specificity Profiling: Ac-IEPD-AFC aids in profiling substrate specificity for caspase family members and related proteases. Its defined peptide sequence allows for comparative analysis of cleavage efficiency across different enzymes, supporting studies aimed at elucidating protease-substrate interactions, substrate preference, and the functional roles of individual caspases within complex biological systems.
Cell-Based Assays: The substrate is also applicable in cell-based assay formats, where it serves as a sensitive reporter for monitoring caspase-8 activity within live or lysed cells. This capability enables researchers to correlate biochemical activity with phenotypic outcomes, such as cell viability or morphological changes, thereby providing a comprehensive view of apoptosis induction and progression under various experimental conditions.
1. Implications of ligand-receptor binding kinetics on GLP-1R signalling
2. Urinary Metabolites Associated with Blood Pressure on a Low-or High-Sodium Die
4. High fat diet and GLP-1 drugs induce pancreatic injury in mice
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More