Boc-DL-Leu-DL-Ser-DL-xiThr-DL-Arg-AMC is a mixed-chirality peptide featuring an AMC fluorophore that enables sensitive detection of enzymatic turnover. The Boc-protected N-terminus stabilizes the substrate during synthetic manipulation. Researchers use it to examine protease specificity, catalytic behavior, and polarity-driven interactions. Its diverse residue set enhances conformational variability.
CAT No: R2260
CAS No:73554-93-5
Synonyms/Alias:Boc-leu-ser-thr-arg-mca;73554-93-5;Boc-Leu-Ser-Thr-Arg-AMC trifluoroacetate salt;Boc-Leu-Ser-Thr-Arg-7-amido-4-methylcoumarin;Boc-Leu-Ser-Thr-Arg-AMC;MLS-3112-V;Boc-Leu-Ser-Thr-Arg-7-amido-4-methylcoumarin, protein C substrate;t-Butyloxycarbonyl-L-Leucyl-L-Seryl-L-Threonyl-L-Arginine 4-Methyl-Coumaryl-7-Amide; Boc-Leu-Ser-Thr-Arg-MCA; Boc-LSTR-AMC;
Boc-DL-Leu-DL-Ser-DL-xiThr-DL-Arg-AMC is a synthetic peptide substrate designed for advanced biochemical research and enzymatic activity assays. Characterized by its protected N-terminus and the incorporation of the fluorogenic 7-amino-4-methylcoumarin (AMC) moiety at the C-terminus, this compound offers unique utility in the detection and quantification of protease activity. The combination of both hydrophobic and hydrophilic amino acid residues within its sequence allows for selective interactions with various enzymes, making it a versatile tool in the study of substrate specificity. The presence of the Boc protecting group ensures enhanced stability during storage and handling, while the AMC label provides a sensitive readout through fluorescence upon enzymatic cleavage. Researchers value this substrate for its adaptability in high-throughput screening platforms and its compatibility with a broad range of buffer conditions, supporting diverse experimental designs in academic and industrial laboratories.
Protease Activity Assays: Boc-DL-Leu-DL-Ser-DL-xiThr-DL-Arg-AMC serves as an indispensable substrate in the assessment of protease activity, particularly serine and arginine-specific proteases. Upon enzymatic cleavage at the peptide bond adjacent to the AMC group, a highly fluorescent signal is generated, enabling sensitive and quantitative measurement of enzyme kinetics. This feature is especially crucial for researchers aiming to characterize enzyme inhibitors, determine kinetic parameters, or optimize reaction conditions in a reproducible and efficient manner.
Enzyme Specificity Profiling: In enzymology research, this peptide substrate is widely used to profile the specificity of various proteases. By analyzing the cleavage patterns and rates in the presence of different enzymes, scientists can elucidate substrate preferences and map the active sites of target enzymes. Such specificity profiling is essential for understanding enzyme function at the molecular level and for guiding the development of selective inhibitors or engineered enzymes with tailored activity profiles.
High-Throughput Screening: The AMC-labeled peptide is ideally suited for high-throughput screening (HTS) applications, where rapid and parallel analysis of multiple samples is required. Its robust fluorescent readout enables automated detection systems to efficiently identify active compounds, inhibitors, or modulators of protease function. This accelerates the drug discovery process and supports large-scale screening efforts in pharmaceutical research and biotechnology development.
Biochemical Pathway Elucidation: Researchers employ Boc-DL-Leu-DL-Ser-DL-xiThr-DL-Arg-AMC to investigate the roles of proteases within complex biochemical pathways. By monitoring substrate cleavage in cell lysates or purified enzyme preparations, it is possible to track proteolytic events and map signaling cascades involving specific protease families. This approach aids in unraveling the regulatory mechanisms underlying cellular processes such as apoptosis, cell migration, and protein turnover.
Educational and Training Applications: In academic settings, the fluorogenic peptide substrate is frequently utilized as a teaching tool in laboratory courses focused on enzymology and protein chemistry. Its clear and measurable fluorescence response provides students with practical experience in enzyme assay design, data interpretation, and kinetic analysis, fostering a deeper understanding of fundamental biochemical principles.
Peptide substrate Boc-DL-Leu-DL-Ser-DL-xiThr-DL-Arg-AMC thus offers a multifaceted platform for advancing research in enzymology, drug discovery, and biochemical education. Its sensitive AMC-based detection system, combined with a thoughtfully designed peptide sequence, enables precise monitoring of proteolytic activity across a variety of experimental contexts. Whether supporting the identification of novel enzyme inhibitors, contributing to the mapping of cellular signaling networks, or enhancing educational experiences in the laboratory, this synthetic substrate stands as a valuable resource for scientists seeking reliable and innovative solutions in peptide-based research.
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