c-Myc Peptide mimics a key transactivation domain of the c-Myc protein involved in PPI interactions. Acidic, aromatic, and hydrophobic residues contribute to structural adaptability. Researchers study its binding to transcriptional coactivators and dynamic structural features. Applications include PPI analysis, transcription-factor modeling, and inhibitor design.
CAT No: R2390
CAS No:145646-22-6
Synonyms/Alias:c-Myc Peptide;145646-22-6;(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-5-amino-2-[[(2S)-2-amino-4-carboxybutanoyl]amino]-5-oxopentanoyl]amino]hexanoyl]amino]-4-methylpentanoyl]amino]-3-methylpentanoyl]amino]-3-hydroxypropanoyl]amino]-4-carboxybutanoyl]amino]-4-carboxybutanoyl]amino]-3-carboxypropanoyl]amino]-4-methylpentanoic acid;G90894;
c-Myc Peptide is a synthetic peptide corresponding to a specific sequence derived from the c-Myc protein, a well-characterized transcription factor involved in the regulation of cell proliferation, differentiation, and apoptosis. Widely recognized in molecular biology and biochemistry research, this peptide serves as a valuable tool for studying protein-protein interactions, signal transduction pathways, and cellular regulatory mechanisms. Its unique amino acid composition enables researchers to utilize it in a variety of experimental protocols, including antibody production, immunoassays, and functional studies. The c-Myc Peptide is often employed as an epitope tag, facilitating the detection, purification, and characterization of recombinant proteins in both prokaryotic and eukaryotic expression systems.
Antibody Generation: c-Myc Peptide is extensively used as an immunogen for the generation of highly specific polyclonal and monoclonal antibodies. By conjugating the peptide to a carrier protein and immunizing host animals, researchers can produce antibodies that recognize the c-Myc epitope with high affinity. These antibodies are indispensable tools in Western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assays, enabling the sensitive detection of c-Myc-tagged proteins in complex biological samples. The use of this peptide for antibody production not only streamlines experimental workflows but also enhances the reproducibility and specificity of protein analysis.
Epitope Tagging and Protein Purification: As an epitope tag, c-Myc Peptide is genetically fused to recombinant proteins to facilitate their detection and purification. The c-Myc tag allows for the use of anti-c-Myc antibodies in affinity chromatography, immunoprecipitation, and co-immunoprecipitation experiments, simplifying the isolation of tagged proteins from cell lysates. This approach is particularly advantageous for purifying proteins that lack suitable endogenous markers or for studying protein complexes under native conditions. The small size of the c-Myc tag minimizes interference with protein structure and function, making it a preferred choice for both in vitro and in vivo applications.
Protein-Protein Interaction Studies: Researchers leverage c-Myc Peptide in the investigation of protein-protein interactions, especially when combined with other tagging systems in co-immunoprecipitation or pull-down assays. By tagging one protein with c-Myc and another with a different epitope, scientists can map interaction networks, identify binding partners, and characterize the dynamics of multiprotein complexes. The specificity of anti-c-Myc antibodies ensures minimal background and high signal-to-noise ratios, supporting the elucidation of intricate cellular signaling pathways and regulatory mechanisms.
Assay Development and Standardization: The synthetic nature and well-defined sequence of c-Myc Peptide make it an excellent calibrator or positive control in a range of immunological assays. It is frequently used to validate and standardize assay conditions, optimize antibody concentrations, and assess assay sensitivity and specificity. By incorporating this peptide into assay development workflows, laboratories can achieve consistent and reproducible results across multiple experiments, enhancing the reliability of data interpretation and downstream analyses.
Cellular Localization and Expression Analysis: Utilizing c-Myc Peptide as a tag enables detailed studies of protein expression, intracellular localization, and trafficking. Fluorescently labeled anti-c-Myc antibodies can be employed in immunofluorescence microscopy to visualize the spatial distribution of c-Myc-tagged proteins within cells, providing insights into their functional roles and subcellular compartmentalization. This application is particularly valuable for dissecting the dynamics of protein movement, post-translational modifications, and interactions with cellular organelles in live or fixed samples. In summary, the versatility and reliability of c-Myc Peptide make it an indispensable reagent in life sciences research, supporting a broad spectrum of experimental approaches from antibody production to advanced cellular imaging.
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