CEF6 is a 9-aa-long peptide corresponding to aa 418-426 of the influenza A virus (H1N1) nucleocapsid protein.
CAT No: R1282
CAS No:913545-15-0
Synonyms/Alias:CEF6;913545-15-0;CID 146160158;4-[[6-amino-1-[[1-[[1-[[1-[(1-carboxy-3-methylsulfanylpropyl)amino]-3-methyl-1-oxobutan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-1-oxohexan-2-yl]amino]-3-[[2-[[2-[(2-amino-4-methylpentanoyl)amino]-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-oxobutanoic acid;H-DL-Leu-DL-Phe-DL-Phe-DL-Asp-DL-Lys-DL-xiThr-DL-xiThr-DL-Val-DL-Met-OH;DA-72061;
CEF6 is a synthetic peptide pool composed of defined peptide sequences derived from cytomegalovirus (CMV), Epstein-Barr virus (EBV), and influenza virus. As a complex mixture of immunologically relevant peptides, CEF6 serves as a critical tool in immunological research, particularly for studying antigen-specific T cell responses. Its design is based on well-characterized viral epitopes that are recognized by human leukocyte antigen (HLA) class I-restricted cytotoxic T lymphocytes. This reagent is widely employed in research settings to assess cellular immune function, calibrate immune monitoring assays, and validate immunological detection systems, making it an essential component in the field of cellular immunology and vaccine development.
Immunological assay calibration: CEF6 is frequently utilized as a positive control in the calibration and validation of T cell-based immunological assays, such as enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICS) assays. By providing a standardized set of viral epitopes that reliably stimulate CD8+ T cell responses in a significant proportion of human donors, it enables researchers to confirm assay sensitivity, reproducibility, and overall performance. This function is especially crucial for laboratories developing or standardizing high-throughput immune monitoring platforms.
T cell functional analysis: Researchers employ this peptide pool to probe the functionality of antigen-specific cytotoxic T lymphocytes in peripheral blood mononuclear cell (PBMC) samples. By exposing PBMCs to the defined viral epitopes contained within the mixture, investigators can measure cytokine production, degranulation, and other activation markers, thereby gaining insights into the magnitude and quality of cellular immune responses. This approach is instrumental in studies of immune competence, vaccine immunogenicity, and immune monitoring in various research contexts.
Quality control of immune reagents: In the production and quality assurance of immunological reagents, CEF6 serves as a benchmark for verifying the biological activity and consistency of T cell detection kits and flow cytometry-based assays. Its well-defined composition and established immunogenicity make it a preferred standard for lot-to-lot comparison and performance validation, ensuring that immune monitoring reagents maintain high reliability and accuracy over time.
HLA-restricted epitope mapping: The defined peptide sequences within this pool are valuable for dissecting HLA-restricted antigen recognition patterns. By analyzing T cell responses to individual or pooled peptides, researchers can map specific HLA-epitope interactions, facilitating the identification of immunodominant viral epitopes and advancing the understanding of cellular immune specificity. This application is particularly relevant in basic immunology research, epitope discovery, and the development of personalized immunotherapeutic strategies.
Vaccine research and development: CEF6 is widely applied in preclinical vaccine studies to evaluate the induction of robust, antigen-specific T cell responses following immunization. By serving as a standardized reference for assessing cellular immunity to viral antigens, it enables comparative analysis of immune responses elicited by candidate vaccines or immunomodulatory interventions. This use supports the rational design and optimization of vaccine formulations targeting viral pathogens and contributes to the advancement of immunoprophylactic research.
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