CELL FREE" AMINO ACID MIX (20 AA) (U-13C+; U-15N) contains a defined set of 20 amino acids used as free amino acid building blocks, each present as an isotopically labeled analogue bearing uniformly 13C and 15N enrichment. The mixture includes amino acids with their native amino and carboxyl functional groups (and side-chain functionalities characteristic of the corresponding proteinogenic amino acids), while the isotopic substitution at carbon and nitrogen positions enables mass spectrometric discrimination and tracer readouts without changing the overall amino acid connectivity. In cell-free protein synthesis, metabolic labeling studies, and analytical method development, the labeled amino acid pool functions as a substrate mixture for incorporation into peptides or as an isotopic reference for quantifying incorporation, turnover, and relative abundance in biochemical and chemical biology workflows.
CAT No: L-Iso-0156
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