D-Lys(Z)-Pro-Arg-pNA (diacetate) diacetate is a chromogenic substrate.
CAT No: R1327
CAS No:108963-70-8
Synonyms/Alias:D-Lys(Z)-Pro-Arg-pNA (diacetate);108963-70-8;D-Lys(Z)-Pro-Arg-pNA diacetate;acetic acid;benzyl N-[(5R)-5-amino-6-[(2S)-2-[[(2S)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]carbamoyl]pyrrolidin-1-yl]-6-oxohexyl]carbamate;Spectrozyme PCa;D-Lys(Z)-Pro-Arg-pNAdiacetate;EX-A7847W;HY-P0021A;CS-4603;DA-62947;MS-31401;G14618;
D-Lys(Z)-Pro-Arg-pNA diacetate is a synthetic peptide substrate designed for precise enzymatic studies within the fields of biochemistry and molecular biology. Structurally, it incorporates a D-lysine residue protected by a benzyloxycarbonyl (Z) group, followed by proline and arginine, with a para-nitroanilide (pNA) chromogenic moiety at the C-terminus. The unique arrangement of amino acids and the pNA label enables sensitive detection of proteolytic activity, making this compound a valuable tool for researchers investigating protease specificity, enzyme kinetics, and substrate recognition. Its defined sequence and chromogenic properties support a range of analytical and mechanistic studies, facilitating advancements in protease research and assay development.
Enzyme activity assays: D-Lys(Z)-Pro-Arg-pNA diacetate is widely utilized as a chromogenic substrate for serine proteases, particularly those with trypsin-like or related substrate specificity. Upon enzymatic cleavage at the arginine-pNA bond, the released pNA group generates a measurable yellow color, allowing real-time quantification of proteolytic activity. This feature is instrumental in the characterization of enzyme kinetics, assessment of catalytic efficiency, and comparison of protease isoforms under various experimental conditions.
Protease inhibitor screening: The substrate serves as a reliable analytical tool in the evaluation of potential protease inhibitors. By monitoring the rate of pNA release in the presence of candidate compounds, researchers can quantitatively assess inhibitor potency and specificity. This approach is critical for drug discovery programs targeting serine proteases, as well as for the development of novel biochemical probes and functional modulators.
Substrate specificity profiling: The defined sequence of D-Lys(Z)-Pro-Arg-pNA diacetate enables detailed investigation of protease substrate preferences. By comparing the cleavage rates of this substrate with those of related analogs, scientists can elucidate the structural determinants of enzyme-substrate recognition. Such studies provide valuable insights into protease active site architecture, substrate binding modes, and the molecular basis of enzymatic selectivity.
Biochemical pathway elucidation: In complex biological samples, the substrate can be employed to monitor protease activity as a functional readout of specific signaling or metabolic pathways. By tracking enzymatic conversion in cell lysates, tissue extracts, or purified systems, researchers gain quantitative data on protease function, regulation, and involvement in physiological or pathological processes. This application supports the mapping of proteolytic cascades and the identification of key regulatory nodes.
High-throughput screening applications: The robust colorimetric response and straightforward assay format of D-Lys(Z)-Pro-Arg-pNA diacetate make it well-suited for use in automated, high-throughput platforms. Laboratories engaged in large-scale screening of enzyme modulators, substrate libraries, or environmental samples benefit from the substrate's compatibility with microplate readers and rapid data acquisition. This efficiency accelerates discovery workflows and supports the generation of reproducible, quantitative results across diverse research settings.
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