Fmoc-Gly-OH-1-13C is an Fmoc-protected glycine derivative bearing a 13C label at the glycine carbon-1 position, placing it in the class of isotopically labeled, amino acid building blocks for peptide-related synthesis. The molecule contains an N-(9H-fluorenylmethoxycarbonyl) protecting group on the amino functionality and a free carboxylic acid (-COOH) for coupling, while the glycine side chain is minimal (-CH2-) and the isotopic enrichment provides a defined carbon labeling site for spectroscopic and mass-based tracking. It is used as a labeled precursor in solid-phase or solution-phase assembly of peptides and amino acid derivatives, supporting structure-property studies, isotopic tracing, and analytical method development where incorporation of a defined 13C label at the glycine position is required.
CAT No: HB00065
Fmoc-Gly-OH-1-13C is a glycine-based, Fmoc-protected amino acid building block bearing a stable 13C label at the carboxyl carbon (1-13C). This labeled protected amino acid is designed for incorporation into peptide synthesis workflows where the carbonyl carbon label enables downstream mass spectrometry-based tracking and quantitation. Researchers use this reagent to generate isotopically defined peptide standards and labeled peptide segments for analytical method development, proteomics-oriented quantification, and chemical biology studies requiring defined isotope shifts.
1. Isotopic Peptide Standards
Fmoc-Gly-OH-1-13C is used by analytical and proteomics laboratories to prepare isotope-resolved peptide standards in which glycine's carbonyl carbon contributes a predictable mass shift. In LC-MS workflows, labeled peptide segments help improve identification confidence and support calibration or verification of chromatographic and ionization behavior for glycine-containing peptides. This is particularly valuable when glycine appears in tryptic or enzymatic peptides and when isotope-coded internal standards are needed to reduce run-to-run variability in quantitative experiments.
2. Proteomics Quantification Support
Fmoc-Gly-OH-1-13C serves as a building block for synthesizing labeled peptides used as reference materials in targeted proteomics and method optimization. By incorporating a defined 13C label into peptide sequences, researchers can evaluate fragment ion patterns and retention time alignment for glycine-containing targets, supporting more robust assay development for selected reaction monitoring (SRM/PRM) or other targeted MS strategies. The Fmoc-protected form also aligns with established peptide synthesis pipelines used to generate sequence-specific standards rather than relying on post-synthetic labeling.
3. Chemical Biology Peptide Tracing
Fmoc-Gly-OH-1-13C is employed in chemical biology and labeling studies where isotopically defined peptides are required to track peptide handling, processing, or analytical recovery across experimental workflows. Labeled glycine-containing motifs can be incorporated into custom peptide probes or peptide substrates to create materials with defined isotope signatures for MS readout. This approach is commonly used when researchers need unambiguous discrimination between closely related peptide species or when they want to monitor specific peptide fragments in complex mixtures.
4. Labeled Segment Synthesis
Fmoc-Gly-OH-1-13C is used to assemble glycine-containing peptide segments for downstream applications that require a controlled, site-specific isotope label within the peptide backbone. Peptide synthesis teams often select this reagent when they need a single labeled residue to propagate a known isotopic signature into the final peptide standard, reference material, or research reagent. The Fmoc protection supports routine solid-phase peptide synthesis workflows, enabling reproducible incorporation of the 13C-labeled glycine into larger constructs used for analytical and research purposes.
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