Keratin, type II cytoskeletal 8
Keratin 8 variant, partial (253-264) is a synthetic peptide fragment derived from the intermediate filament protein keratin 8, specifically encompassing amino acid residues 253 to 264. As a member of the keratin family, keratin 8 plays a pivotal role in maintaining the structural integrity of epithelial cells and is widely studied in the context of cytoskeletal dynamics, cell signaling, and tissue organization. The partial sequence represented by this peptide offers a focused tool for investigating region-specific interactions, post-translational modifications, and the functional biology of keratin 8 in both normal and pathological states. Its defined sequence and biochemical properties make it a valuable resource for researchers aiming to dissect the molecular underpinnings of keratin-based processes.
Epitope mapping: The defined amino acid sequence of this peptide fragment makes it highly suitable for epitope mapping studies. Researchers can use the 253-264 region to identify and characterize antibody-binding sites within keratin 8, facilitating the development or validation of monoclonal and polyclonal antibodies. Such mapping is essential for the creation of specific immunoreagents used in immunohistochemistry, Western blotting, and other immunodetection methods, enabling precise localization and quantification of keratin 8 in various biological samples.
Protein-protein interaction analysis: As an isolated segment of keratin 8, this peptide serves as a model substrate for studying protein-protein interactions that are specific to the 253-264 region. By employing the peptide in pull-down assays, affinity chromatography, or surface plasmon resonance experiments, researchers can probe how binding partners, such as chaperones, regulatory proteins, or cytoskeletal components, recognize and interact with this segment. Insights gained from such studies contribute to a deeper understanding of intermediate filament assembly and the regulatory mechanisms governing cellular architecture.
Phosphorylation and post-translational modification research: The 253-264 region of keratin 8 is known to harbor potential sites for phosphorylation and other post-translational modifications. Synthetic access to this peptide fragment allows researchers to investigate the biochemical consequences of such modifications in vitro, using kinase assays or mass spectrometry-based analyses. These studies elucidate the regulatory roles of site-specific modifications in keratin 8 function, cytoskeletal organization, and signal transduction pathways.
Analytical assay development: The peptide can be employed as a standard or control in the development of quantitative analytical assays, such as enzyme-linked immunosorbent assays (ELISA) or mass spectrometric methods. Its defined sequence and biochemical stability enable its use in calibrating detection systems, validating assay specificity, and optimizing protocols for the sensitive and accurate measurement of keratin 8 or its variants in research samples. This supports robust quantification and comparative studies across experimental conditions.
Structural and conformational studies: The partial sequence offers a tractable model for investigating the secondary structure and conformational properties of keratin 8 subdomains. Circular dichroism spectroscopy, nuclear magnetic resonance (NMR), or computational modeling can be applied to this peptide to assess its folding behavior and structural motifs. Such studies provide valuable data on the intrinsic properties of keratin 8 segments, informing hypotheses about filament assembly, mechanical resilience, and the impact of sequence variation on protein function.
1. High fat diet and GLP-1 drugs induce pancreatic injury in mice
2. C-Peptide replacement therapy and sensory nerve function in type 1 diabetic neuropathy
If you have any peptide synthesis requirement in mind, please do not hesitate to contact us at . We will endeavor to provide highly satisfying products and services.
Creative Peptides is a trusted CDMO partner specializing in high-quality peptide synthesis, conjugation, and manufacturing under strict cGMP compliance. With advanced technology platforms and a team of experienced scientists, we deliver tailored peptide solutions to support drug discovery, clinical development, and cosmetic innovation worldwide.
From custom peptide synthesis to complex peptide-drug conjugates, we provide flexible, end-to-end services designed to accelerate timelines and ensure regulatory excellence. Our commitment to quality, reliability, and innovation has made us a preferred partner across the pharmaceutical, biotechnology, and personal care industries.
Read More