LONG R3 IGF-I is a recombinant analogue of human insulin-like growth factor-I (IGF-I), specially designed to improve cell culture performance. It has higher biological activity than insulin or natural IGF-I in vitro and has been shown to significantly increase the production of recombinant protein. It is an ideal choice for research and large-scale culture systems (serum-free or low-level serum applications).
CAT No: 10-101-261
CAS No:143045-27-6
Synonyms/Alias:long R3 igf-I;LONGR3IGF-I MEDIA GRADE;LONG? R3 IGF-I human ;LONG R3 IGF-I RECOMBINANT ANALOG
Chemical Name:LONG R3 IGF-I RECOMBINANT ANALOG
LONG R3 IGF-1 (human) is a synthetic peptide analog of human insulin-like growth factor 1, engineered with specific amino acid substitutions and an extended N-terminal sequence to enhance its biological stability and receptor affinity. As a member of the IGF family, this modified protein plays a significant role in cellular growth, differentiation, and metabolic regulation, making it a valuable tool for a wide range of research applications. Its resistance to binding by insulin-like growth factor-binding proteins (IGFBPs) and increased half-life compared to native IGF-1 allow for more consistent and potent activity in experimental systems. Researchers utilize this peptide to investigate signaling pathways, cellular responses, and biochemical mechanisms relevant to growth factor biology and metabolic regulation.
Cell Culture Supplementation: In vitro, LONG R3 IGF-1 is widely employed to stimulate proliferation, survival, and differentiation of various mammalian cell types, including primary cells and established cell lines. Its enhanced bioactivity and reduced affinity for IGFBPs allow it to maintain higher effective concentrations in culture media, supporting robust cell growth and viability. This makes the analog particularly useful in serum-free or low-serum culture conditions, where it can substitute for or augment native growth factors, ensuring reproducible and sustained cellular responses in experimental protocols.
Signal Transduction Research: The analog's high affinity for the IGF-1 receptor and its resistance to sequestration by binding proteins render it an optimal probe for dissecting IGF-mediated signaling pathways. Researchers use LONG R3 IGF-1 to activate downstream effectors such as PI3K/Akt and MAPK, facilitating studies on cell cycle progression, apoptosis inhibition, and metabolic regulation. Its defined and potent activity enables precise modulation of signaling cascades, aiding in the elucidation of growth factor-dependent molecular mechanisms in diverse biological models.
Protein Synthesis and Metabolic Studies: As a potent stimulator of anabolic processes, LONG R3 IGF-1 is frequently utilized to investigate protein synthesis, glucose uptake, and other metabolic events in cultured cells and tissue explants. Its ability to mimic and amplify the effects of endogenous IGF-1 makes it an indispensable tool for examining the regulation of muscle hypertrophy, energy metabolism, and nutrient signaling. Researchers leverage these properties to explore cellular adaptation, metabolic flux, and biosynthetic pathways under controlled experimental conditions.
Peptide Engineering and Functional Analysis: The unique structural modifications of LONG R3 IGF-1 offer valuable insights for peptide engineering and structure-activity relationship studies. By comparing the biological effects of this analog with those of native IGF-1 or other variants, scientists can delineate the contributions of specific amino acid residues to receptor binding, stability, and functional potency. Such comparative analyses inform the rational design of next-generation peptide therapeutics and research reagents with tailored activity profiles.
Assay Development and Analytical Validation: The predictable and robust activity of LONG R3 IGF-1 supports its use as a standard or positive control in various bioassays, including receptor binding, cell proliferation, and functional response assays. Its consistent performance enables accurate calibration and validation of assay systems, facilitating the quantification of IGF-1 activity and the assessment of pathway modulation. This application is especially valuable in high-throughput screening, quality control, and the development of new analytical methodologies targeting growth factor signaling.
1. Urinary Metabolites Associated with Blood Pressure on a Low-or High-Sodium Die
2. Autoinhibition and phosphorylation-induced activation of phospholipase C-γ isozymes
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